Chloride as a Beneficial Macronutrient in Higher Plants: New Roles and Regulation

• Main Authors: José M. Colmenero-Flores, Juan D. Franco-Navarro, Paloma Cubero-Font, Procopio Peinado-Torrubia, Miguel A. Rosales
• Format: Article
• Language: English
• Published: MDPI AG 2019-09-01
• Series: International Journal of Molecular Sciences
• Subjects: chloride homeostasis; beneficial macronutrient; ion transport; anion channels; plant nutrition; turgor; nitrate; water balance; WUE; NUE; stress
• Online Access: https://www.mdpi.com/1422-0067/20/19/4686

Chloride (Cl^&#8722;) has traditionally been considered a micronutrient largely excluded by plants due to its ubiquity and abundance in nature, its antagonism with nitrate (NO₃^&#8722;), and its toxicity when accumulated at high concentrations. In recent years, there has been a paradigm shift in this regard since Cl^&#8722; has gone from being considered a harmful ion, accidentally absorbed through NO₃^&#8722; transporters, to being considered a beneficial macronutrient whose transport is finely regulated by plants. As a beneficial macronutrient, Cl^&#8722; determines increased fresh and dry biomass, greater leaf expansion, increased elongation of leaf and root cells, improved water relations, higher mesophyll diffusion to CO₂, and better water- and nitrogen-use efficiency. While optimal growth of plants requires the synchronic supply of both Cl^&#8722; and NO₃^&#8722; molecules, the NO₃^&#8722;/Cl^&#8722; plant selectivity varies between species and varieties, and in the same plant it can be modified by environmental cues such as water deficit or salinity. Recently, new genes encoding transporters mediating Cl^&#8722; influx (<i>ZmNPF6.4</i> and <i>ZmNPF6.6</i>), Cl^&#8722; efflux (<i>AtSLAH3</i> and <i>AtSLAH1</i>), and Cl^&#8722; compartmentalization (<i>AtDTX33</i>, <i>AtDTX35</i>, <i>AtALMT4,</i> and <i>GsCLC2</i>) have been identified and characterized. These transporters have proven to be highly relevant for nutrition, long-distance transport and compartmentalization of Cl^&#8722;, as well as for cell turgor regulation and stress tolerance in plants.