Genotypic profile of Listeria monocytogenes isolated in refrigerated chickens in southern Rio Grande do Sul, Brazil

• Main Authors: Karla Sequeira Mendonça, Geovana Brenner Michael, Élen Silveira Nalério, Marcelo Mendonça, Marisa Ribeiro Itapema Cardoso, Wladimir Padilha da Silva
• Format: Article
• Language: English
• Published: Universidade Federal de Santa Maria 2016-01-01
• Series: Ciência Rural
• Subjects: contaminação; patógeno; prevalência; sorotipagem
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100132&lng=en&tlng=en

ABSTRACT: Listeria monocytogenes is of notable concern to the food industry, due to its ubiquitous nature and ability to grow in adverse conditions. This study aimed to determine the genotypic profile of L. monocytogenes strains isolated from refrigerated chickens marketed in the southern part of Rio Grande do Sul, Brazil. The strains of L. monocytogenes isolated were characterized by serotyping and Pulsed Field Gel Electrophoresis (PFGE). Three different serotypes (1/2a, 1/2b and 4e) were evaluated by PFGE, and the macrorestriction patterns utilizing enzymes AscI and ApaI, revealed five different pulsotypes. The presence of such varied genotypic profiles demonstrates the prevalence of L. monocytogenes contamination of chicken processing environments, which combined with ineffective cleaning procedures, allowing the survival, adaptation and proliferation of these pathogens, not only in the processing environment, but also in local grocery stores.