Purification of cross-linked RNA-protein complexes by phenol-toluol extraction
RNA binding proteins are important regulators of RNA function. Here the authors describe a method for isolation of RNA-protein complexes that does not rely on a specific RNA sequence or motif, and demonstrate the approach by providing the global RNA-bound proteomes of human HEK293 cells and Salmonel...
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Bibliographic Details
| Main Authors: |
Erika C. Urdaneta,
Carlos H. Vieira-Vieira,
Timon Hick,
Hans-Herrmann Wessels,
Davide Figini,
Rebecca Moschall,
Jan Medenbach,
Uwe Ohler,
Sander Granneman,
Matthias Selbach,
Benedikt M. Beckmann |
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| Format: | Article
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| Language: | English |
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| Published: |
Nature Publishing Group
2019-03-01
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| Series: | Nature Communications
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| Online Access: | https://doi.org/10.1038/s41467-019-08942-3
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