Purification of cross-linked RNA-protein complexes by phenol-toluol extraction

Purification of cross-linked RNA-protein complexes by phenol-toluol extraction

RNA binding proteins are important regulators of RNA function. Here the authors describe a method for isolation of RNA-protein complexes that does not rely on a specific RNA sequence or motif, and demonstrate the approach by providing the global RNA-bound proteomes of human HEK293 cells and Salmonel...

Full description

Bibliographic Details
Main Authors: Erika C. Urdaneta, Carlos H. Vieira-Vieira, Timon Hick, Hans-Herrmann Wessels, Davide Figini, Rebecca Moschall, Jan Medenbach, Uwe Ohler, Sander Granneman, Matthias Selbach, Benedikt M. Beckmann
Format: Article
Language:English
Published: Nature Publishing Group 2019-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-08942-3

Internet

https://doi.org/10.1038/s41467-019-08942-3

Similar Items