Generation of a human induced pluripotent stem cell line from a 65-year old healthy female donor with Chinese Han genetic background

Peripheral blood mononuclear cells (PBMC) were collected from a 65-year old healthy woman with Chinese Han genetic background. The PBMCs were reprogrammed with the human OKSM transcription factors using the non-integrating episomal vector system. The transgene-free iPSC showed pluripotency verified...

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Main Authors:	Ying Wang, Jing Zhang, Yue Zhang, Danyi Huang, Jian Zhao, Gang Li, Ying Lei
Format:	Article
Language:	English
Published:	Elsevier 2017-10-01
Series:	Stem Cell Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S1873506117301629

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spelling	doaj-4c0e146df5ab427291e4cde5c7d53fb52020-11-24T22:56:16ZengElsevierStem Cell Research1873-50611876-77532017-10-0124C333510.1016/j.scr.2017.08.008Generation of a human induced pluripotent stem cell line from a 65-year old healthy female donor with Chinese Han genetic backgroundYing Wang0Jing Zhang1Yue Zhang2Danyi Huang3Jian Zhao4Gang Li5Ying Lei6Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaIxCell Biotechnology Co., Ltd, Shanghai, ChinaStem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaDepartment of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaStem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaPeripheral blood mononuclear cells (PBMC) were collected from a 65-year old healthy woman with Chinese Han genetic background. The PBMCs were reprogrammed with the human OKSM transcription factors using the non-integrating episomal vector system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously toward the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. The iPSC line can be used as control in disease mechanism studies.http://www.sciencedirect.com/science/article/pii/S1873506117301629
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Ying Wang Jing Zhang Yue Zhang Danyi Huang Jian Zhao Gang Li Ying Lei
spellingShingle	Ying Wang Jing Zhang Yue Zhang Danyi Huang Jian Zhao Gang Li Ying Lei Generation of a human induced pluripotent stem cell line from a 65-year old healthy female donor with Chinese Han genetic background Stem Cell Research
author_facet	Ying Wang Jing Zhang Yue Zhang Danyi Huang Jian Zhao Gang Li Ying Lei
author_sort	Ying Wang
title	Generation of a human induced pluripotent stem cell line from a 65-year old healthy female donor with Chinese Han genetic background
title_short	Generation of a human induced pluripotent stem cell line from a 65-year old healthy female donor with Chinese Han genetic background
title_full	Generation of a human induced pluripotent stem cell line from a 65-year old healthy female donor with Chinese Han genetic background
title_fullStr	Generation of a human induced pluripotent stem cell line from a 65-year old healthy female donor with Chinese Han genetic background
title_full_unstemmed	Generation of a human induced pluripotent stem cell line from a 65-year old healthy female donor with Chinese Han genetic background
title_sort	generation of a human induced pluripotent stem cell line from a 65-year old healthy female donor with chinese han genetic background
publisher	Elsevier
series	Stem Cell Research
issn	1873-5061 1876-7753
publishDate	2017-10-01
description	Peripheral blood mononuclear cells (PBMC) were collected from a 65-year old healthy woman with Chinese Han genetic background. The PBMCs were reprogrammed with the human OKSM transcription factors using the non-integrating episomal vector system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously toward the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. The iPSC line can be used as control in disease mechanism studies.
url	http://www.sciencedirect.com/science/article/pii/S1873506117301629
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Generation of a human induced pluripotent stem cell line from a 65-year old healthy female donor with Chinese Han genetic background

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