The Curcumin Analogue, MS13 (1,5-Bis(4-hydroxy-3- methoxyphenyl)-1,4-pentadiene-3-one), Inhibits Cell Proliferation and Induces Apoptosis in Primary and Metastatic Human Colon Cancer Cells

The Curcumin Analogue, MS13 (1,5-Bis(4-hydroxy-3- methoxyphenyl)-1,4-pentadiene-3-one), Inhibits Cell Proliferation and Induces Apoptosis in Primary and Metastatic Human Colon Cancer Cells

The cytotoxic and apoptotic effects of turmeric (Curcuma longa) on colon cancer have been well documented but specific structural modifications of curcumin have been shown to possess greater growth-suppressive potential on colon cancer than curcumin. Therefore, the aim of this study is to identify t...

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Main Authors: Nor Isnida Ismail, Iekhsan Othman, Faridah Abas, Nordin H. Lajis, Rakesh Naidu
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Molecules
Subjects:
curcumin analogue
diarylpentanoid (DAP)
colon cancer
cell proliferation
apoptosis
1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one
Online Access:https://www.mdpi.com/1420-3049/25/17/3798
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spelling doaj-4bfe2b0c139e4bb3a1e2dbf413cb9bf52020-11-25T03:38:39ZengMDPI AGMolecules1420-30492020-08-01253798379810.3390/molecules25173798The Curcumin Analogue, MS13 (1,5-Bis(4-hydroxy-3- methoxyphenyl)-1,4-pentadiene-3-one), Inhibits Cell Proliferation and Induces Apoptosis in Primary and Metastatic Human Colon Cancer CellsNor Isnida Ismail0Iekhsan Othman1Faridah Abas2Nordin H. Lajis3Rakesh Naidu4Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 47500, Selangor, MalaysiaUniKL MESTECH, A1-1 Jalan TKS1, Taman Kajang Sentral, Kajang 43000, MalaysiaLaboratory of Natural Products, Faculty of Science, University Putra Malaysia, Serdang 43400, MalaysiaLaboratory of Natural Products, Faculty of Science, University Putra Malaysia, Serdang 43400, MalaysiaJeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 47500, Selangor, MalaysiaThe cytotoxic and apoptotic effects of turmeric (Curcuma longa) on colon cancer have been well documented but specific structural modifications of curcumin have been shown to possess greater growth-suppressive potential on colon cancer than curcumin. Therefore, the aim of this study is to identify the anti-cancer properties of curcumin analogue-MS13, a diarylpentanoid on the cytotoxicity, anti-proliferative and apoptotic activity of primary (SW480) and metastatic (SW620) human colon cancer cells. A cell viability assay showed that MS13 has greater cytotoxicity effect on SW480 (EC<sub>50</sub>: 7.5 ± 2.8 µM) and SW620 (EC<sub>50</sub>: 5.7 ± 2.4 µM) compared to curcumin (SW480, EC<sub>50</sub>: 30.6 ± 1.4 µM) and SW620, EC<sub>50</sub>: 26.8 ± 2.1 µM). Treatment with MS13 at two different doses 1X EC<sub>50</sub> and 2X EC<sub>50</sub> suppressed the colon cancer cells growth with lower cytotoxicity against normal cells. A greater anti-proliferative effect was also observed in MS13 treated colon cancer cells compared to curcumin at 48 and 72 h. Subsequent analysis on the induction of apoptosis showed that MS13 treated cells exhibited morphological features associated with apoptosis. The findings are also consistent with cellular apoptotic activities shown by increased caspase-3 activity and decreased Bcl-2 protein level in both colon cancer cell lines. In conclusion, MS13 able to suppress colon cancer cell growth by inhibiting cell proliferation and induce apoptosis in primary and metastatic human colon cancer cells.https://www.mdpi.com/1420-3049/25/17/3798curcumin analoguediarylpentanoid (DAP)colon cancercell proliferationapoptosis1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one
collection DOAJ
language English
format Article
sources DOAJ
author Nor Isnida Ismail
Iekhsan Othman
Faridah Abas
Nordin H. Lajis
Rakesh Naidu
spellingShingle Nor Isnida Ismail
Iekhsan Othman
Faridah Abas
Nordin H. Lajis
Rakesh Naidu
The Curcumin Analogue, MS13 (1,5-Bis(4-hydroxy-3- methoxyphenyl)-1,4-pentadiene-3-one), Inhibits Cell Proliferation and Induces Apoptosis in Primary and Metastatic Human Colon Cancer Cells
Molecules
curcumin analogue
diarylpentanoid (DAP)
colon cancer
cell proliferation
apoptosis
1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one
author_facet Nor Isnida Ismail
Iekhsan Othman
Faridah Abas
Nordin H. Lajis
Rakesh Naidu
author_sort Nor Isnida Ismail
title The Curcumin Analogue, MS13 (1,5-Bis(4-hydroxy-3- methoxyphenyl)-1,4-pentadiene-3-one), Inhibits Cell Proliferation and Induces Apoptosis in Primary and Metastatic Human Colon Cancer Cells
title_short The Curcumin Analogue, MS13 (1,5-Bis(4-hydroxy-3- methoxyphenyl)-1,4-pentadiene-3-one), Inhibits Cell Proliferation and Induces Apoptosis in Primary and Metastatic Human Colon Cancer Cells
title_full The Curcumin Analogue, MS13 (1,5-Bis(4-hydroxy-3- methoxyphenyl)-1,4-pentadiene-3-one), Inhibits Cell Proliferation and Induces Apoptosis in Primary and Metastatic Human Colon Cancer Cells
title_fullStr The Curcumin Analogue, MS13 (1,5-Bis(4-hydroxy-3- methoxyphenyl)-1,4-pentadiene-3-one), Inhibits Cell Proliferation and Induces Apoptosis in Primary and Metastatic Human Colon Cancer Cells
title_full_unstemmed The Curcumin Analogue, MS13 (1,5-Bis(4-hydroxy-3- methoxyphenyl)-1,4-pentadiene-3-one), Inhibits Cell Proliferation and Induces Apoptosis in Primary and Metastatic Human Colon Cancer Cells
title_sort curcumin analogue, ms13 (1,5-bis(4-hydroxy-3- methoxyphenyl)-1,4-pentadiene-3-one), inhibits cell proliferation and induces apoptosis in primary and metastatic human colon cancer cells
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2020-08-01
description The cytotoxic and apoptotic effects of turmeric (Curcuma longa) on colon cancer have been well documented but specific structural modifications of curcumin have been shown to possess greater growth-suppressive potential on colon cancer than curcumin. Therefore, the aim of this study is to identify the anti-cancer properties of curcumin analogue-MS13, a diarylpentanoid on the cytotoxicity, anti-proliferative and apoptotic activity of primary (SW480) and metastatic (SW620) human colon cancer cells. A cell viability assay showed that MS13 has greater cytotoxicity effect on SW480 (EC<sub>50</sub>: 7.5 ± 2.8 µM) and SW620 (EC<sub>50</sub>: 5.7 ± 2.4 µM) compared to curcumin (SW480, EC<sub>50</sub>: 30.6 ± 1.4 µM) and SW620, EC<sub>50</sub>: 26.8 ± 2.1 µM). Treatment with MS13 at two different doses 1X EC<sub>50</sub> and 2X EC<sub>50</sub> suppressed the colon cancer cells growth with lower cytotoxicity against normal cells. A greater anti-proliferative effect was also observed in MS13 treated colon cancer cells compared to curcumin at 48 and 72 h. Subsequent analysis on the induction of apoptosis showed that MS13 treated cells exhibited morphological features associated with apoptosis. The findings are also consistent with cellular apoptotic activities shown by increased caspase-3 activity and decreased Bcl-2 protein level in both colon cancer cell lines. In conclusion, MS13 able to suppress colon cancer cell growth by inhibiting cell proliferation and induce apoptosis in primary and metastatic human colon cancer cells.
topic curcumin analogue
diarylpentanoid (DAP)
colon cancer
cell proliferation
apoptosis
1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one
url https://www.mdpi.com/1420-3049/25/17/3798
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