K-RAS mutation profile in colorectal carcinoma patients in Sanglah Hospital Denpasar, Bali-Indonesia

• Main Authors: Luh Putu Iin Indrayani M, Ni Putu Sriwidyani
• Format: Article
• Language: English
• Published: DiscoverSys 2017-08-01
• Series: Bali Medical Journal
• Subjects: k-ras, mutation, colorectal cancer, bali
• Online Access: https://balimedicaljournal.org/index.php/bmj/article/view/717
• ISSN: 2089-1180; 2302-2914

<p><strong>Background: </strong>Today is an era of personalized medicine where treatment is tailored and personalized according to patient status. Assessment of K-ras mutation is one of the routine molecular examination in colorectal carcinoma, and the result will determine the treatment decision particularly, avoidance of using anti-EGFR in such patients. This study was a preliminary research to determine the profile of <em>K-RAS</em> mutation in colorectal carcinoma in Bali. <strong>Methods: </strong>Formalin-fixed paraffin embedded tissue of 23 colorectal carcinoma patients in Sanglah Hospital which admitted in 2016 were collected from Anatomical Pathology Department and sent to Laboratory of Molecular Pathology of Dr. Cipto Mangunkusumo Hospital and Kalgen for <em>K-RAS</em> mutation examination. All of H-E slides were examined, and the specimen with well-preserved tumor cells were selected. The PCR was performed to determine K-RAS mutational status.  <strong>Result: </strong>From 23 samples, 70% was male, and 30% was female with a mean age 48.3 years. Fourteen cases (60.9%) had K-ras mutation (9 cases with codon 12 mutation, 4 cases with codon 13 mutation, 1 case with codon 59 mutation and 1 case with codon 117 mutation). <strong>Conclusion: </strong>It is found that <em>K-RAS</em> mutation was quite common in colorectal carcinoma in Bali with two most common mutations observed in codon 12 and 13. </p>