The polymorphism of Matrilin-3 (RS77245812) and Interleukin-10 (RS1800872) genes in osteoarthritis patients with arterial hypertension, obesity and type 2 diabetes mellitus

• Main Authors: Larysa P. Sydorchuk, Yulia I. Serdulets, Olexandr I. Fediv, Larysa O. Havrysh, Yana M. Teleki, Andrii R. Sydorchuk, Anna I. Kshanovska, Natalia A. Turubarova-Leunova, Denys O. Lekhai
• Format: Article
• Language: English
• Published: Balkan Medical Union 2017-12-01
• Series: Archives of the Balkan Medical Union
• Subjects: matrilin-3 gene (rs77245812); interleukin- 10 gene (rs1800872); osteoarthritis; risk
• Online Access: http://umbalk.org/wp-content/uploads/2017/12/08.THE-POLYMORPHISM-OF-MATRILIN-3-RS77245812.pdf
• ISSN: 1584-9244; 2558-815X

Introduction. Osteoarthritis (OA) is a multifactorial disorder in which ageing, genetic, hormonal and mechanical factors are major contributors to its onset and progression. Objectives. To evaluate the genotypes frequencies of Matrilin-3 (MATN3, rs77245812) and Interleukin-10 (IL-10, rs1800872) genes polymorphism in OA patients combined with arterial hypertension (AH), abdominal obesity (AO) and type 2 diabetes mellitus (DM2). Methods. The polymorphic variants of genes MATN3 (C908T) and IL-10 (C-592A) were analyzed by PCR in 74 OA patients with AO, AH, DM2 and 25 healthy individuals. Results. Distribution of MATN3 gene polymorphic variants in observed groups was as follows: CC-genotype – in 94.59% OA patients, and in 96.0% of control (p>0.05); CC-genotype – in 5.41% and 4% respectively. The C-allele prevails over T-allele by 94.95% (p<0.001) with preservation of the population equilibrium: among the OA patients – 97.30% vs 2.70% (p<0.001), among the healthy – 98.0% vs 2.0% (p<0.001) accordingly. Distribution of IL-10 gene polymorphic variants in observed groups was as follows: CC-genotype – in 52.7% OA patients vs 68.0% in control (p>0.05); CA-genotype – in 37.84% vs 32.0% (p>0.05) respectively; CC-genotype was found in 9.46% OA patients and none of control. C-allele of IL-10 gene dominated over the A-allele: among patients – 2.52 times (p<0.001), among control group – 5.25 times (p<0.001). The wild C-allele of IL-10 gene dominated 2.96 times (p<0.001) generally in the observed population. Conclusions. Polymorphic variants of genes MATN3 and IL-10 are not additional risk factors of the OA occurrence in the observed population of Northern Bukovina.