Cutin Synthesis in Developing, Field-Grown Apple Fruit Examined by External Feeding of Labelled Precursors

• Main Authors: Yiru Si, Bishnu P. Khanal, Leopold Sauheitl, Moritz Knoche
• Format: Article
• Language: English
• Published: MDPI AG 2021-03-01
• Series: Plants
• Subjects: cutin; wax; oleic acid; palmitic acid; <i>Malus</i> × <i>domestica</i>; cuticle
• Online Access: https://www.mdpi.com/2223-7747/10/3/497
• ISSN: 2223-7747

An intact skin is essential in high-quality apples. Ongoing deposition of cuticular material during fruit development may decrease microcracking. Our objective was to establish a system for quantifying cutin and wax deposition in developing apple fruit. Oleic acid (¹³C and ¹⁴C labelled) and palmitic acid (¹⁴C labelled) were fed to developing apples and the amounts incorporated in the cutin and wax fractions were quantified. The incorporation of ¹⁴C oleic acid (C18) was significantly higher than that of ¹⁴C palmitic acid (C16) and the incorporation in the cutin fraction exceeded that in the wax fraction. The amount of precursor incorporated in the cutin increased asymptotically with time, but the amount in the wax fraction remained about constant. Increasing the concentration of the precursor applied generally increased incorporation. Incorporation in the cutin fraction was high during early development (43 days after full bloom) and decreased towards maturity. Incorporation was higher from a dilute donor solution (infinite dose feeding) than from a donor solution subjected to drying (finite dose feeding) or from perfusion of the precursor by injection. Feeding the skin of a developing apple with oleic acid resulted in significant incorporation in the cutin fraction under both laboratory and field conditions.