Evaluation of Embryos Derived from in vitro Fertilized Oocytes Reconstructed by Meiosis-II Chromosome Transplantation from Aged Mice to Ooplasms of Young Mice

• Main Authors: Abdolhossein Shahverdi, Mansoureh Movahedin, Mojtaba Rezazadeh Valojerdi, Hossein Baharvand
• Format: Article
• Language: English
• Published: Royan Institute (ACECR), Tehran 2010-02-01
• Series: International Journal of Fertility and Sterility
• Subjects: aging; chromosome; nuclear transfer; oocyte; ultrastructure
• Online Access: http://www.ijfs.ir/article_45794_e793c9b79a4707109057d2ebd1bd0071.pdf

Background To assess embryos derived by the transfer of meiosis-II chromosomes (M-II-t) from aged mice oocytes into ooplasms from younger mice to overcome the problem of age-related decline in female fertility. Materials and methods The developmental capacity karyotype and ultrastructure of reconstructed oocytes derived from meiosis-II chromosome transplantation from aged mice into the ooplasms of young mice by piezo-micromanipulation were assessed. Results The survival rate of enucleated young oocytes was 54% and the percent of fertilized reconstructed oocytes was 23%. The rate of embryo development to the two-cell stage after cultivation was 40%. Since 82.4% of the analyzed embryos derived from reconstructed oocytes had condensed nuclei it was not possible to analyze their chromosomal integrity. However 17.6% of analyzable reconstructed old oocyte derived embryos (old-ODEs) had normal diploid sets of chromosomes. Major structural differences were not observed between young old and M-II-t derived two-cell embryos. Conclusion Our findings suggested that ooplasms from younger mice may overcome ageassociated problems in older mice.