Evaluation of Blue Value in different plant materials as a tool for rapid starch determination

In order to determine the concentration of starch in plant materials from the intensity of the blue iodine complex, it is necessary to know the Blue Value (B.V.), which is defined in this paper as the absorbancy of 100 mg of a starch-iodine complex in 100 ml of aqueous solution. An adequate amount o...

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Main Authors: Bogusław Samotus, Joanna Tuz, Elżbieta Doerre
Format: Article
Language:English
Published: Polish Botanical Society 2014-01-01
Series:Acta Societatis Botanicorum Poloniae
Subjects:
Online Access:https://pbsociety.org.pl/journals/index.php/asbp/article/view/1141
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spelling doaj-4b96df664c7d4984badfa658124ba21f2020-11-25T03:24:48ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802014-01-01623-413714110.5586/asbp.1993.020877Evaluation of Blue Value in different plant materials as a tool for rapid starch determinationBogusław Samotus0Joanna Tuz1Elżbieta Doerre2Agricultural University of CracowAgricultural University of CracowAgricultural University of CracowIn order to determine the concentration of starch in plant materials from the intensity of the blue iodine complex, it is necessary to know the Blue Value (B.V.), which is defined in this paper as the absorbancy of 100 mg of a starch-iodine complex in 100 ml of aqueous solution. An adequate amount of plant material is treated with a hot CaCl2 solution for 1/2 hour and the solute is diluted to 25 ml with CaCl2. This basic solution serves to measure absorbancy, as well as for starch determination. The first measurement is done by the dilution of a proper amount of basic solution with water and after adding a diluted iodine-iodide solution the reading of B.V. is taken off. The second measurement is done by the precipitation of a starch iodine complex from a proper amount of the basic solution, which is then purified, destroyed by Na2SO3 solution, and starch is determined by the anthrone method. These two readings serve for the establishing of B.V. for the starch. Once established, B.V. can be used for starch determination in the proper plant material. A high degree of variation of the B.V. was found. The highest B.V. was obtained for wrinkled pea seeds (17.4); walnut, potato, smooth pea and pear gave values from 12.6 to 11.0, common bean and broad bean - 10.3 and 9.7, Triticale, carrot, rye, wheat and garden parsley from 8.7 to 8.0 and maize, oat, normal rice from 7.6 to 6.2. The B.V. for amylose was 25.3, for potato starch 12.4, soluble starch 11.9, wheat starch 8.8 and for Triticale and rye starches, 8.7.https://pbsociety.org.pl/journals/index.php/asbp/article/view/1141Blue Valuestarch determinationstarch-iodine complexstarch in plant materialsstarch dissolution in CaCl2
collection DOAJ
language English
format Article
sources DOAJ
author Bogusław Samotus
Joanna Tuz
Elżbieta Doerre
spellingShingle Bogusław Samotus
Joanna Tuz
Elżbieta Doerre
Evaluation of Blue Value in different plant materials as a tool for rapid starch determination
Acta Societatis Botanicorum Poloniae
Blue Value
starch determination
starch-iodine complex
starch in plant materials
starch dissolution in CaCl2
author_facet Bogusław Samotus
Joanna Tuz
Elżbieta Doerre
author_sort Bogusław Samotus
title Evaluation of Blue Value in different plant materials as a tool for rapid starch determination
title_short Evaluation of Blue Value in different plant materials as a tool for rapid starch determination
title_full Evaluation of Blue Value in different plant materials as a tool for rapid starch determination
title_fullStr Evaluation of Blue Value in different plant materials as a tool for rapid starch determination
title_full_unstemmed Evaluation of Blue Value in different plant materials as a tool for rapid starch determination
title_sort evaluation of blue value in different plant materials as a tool for rapid starch determination
publisher Polish Botanical Society
series Acta Societatis Botanicorum Poloniae
issn 2083-9480
publishDate 2014-01-01
description In order to determine the concentration of starch in plant materials from the intensity of the blue iodine complex, it is necessary to know the Blue Value (B.V.), which is defined in this paper as the absorbancy of 100 mg of a starch-iodine complex in 100 ml of aqueous solution. An adequate amount of plant material is treated with a hot CaCl2 solution for 1/2 hour and the solute is diluted to 25 ml with CaCl2. This basic solution serves to measure absorbancy, as well as for starch determination. The first measurement is done by the dilution of a proper amount of basic solution with water and after adding a diluted iodine-iodide solution the reading of B.V. is taken off. The second measurement is done by the precipitation of a starch iodine complex from a proper amount of the basic solution, which is then purified, destroyed by Na2SO3 solution, and starch is determined by the anthrone method. These two readings serve for the establishing of B.V. for the starch. Once established, B.V. can be used for starch determination in the proper plant material. A high degree of variation of the B.V. was found. The highest B.V. was obtained for wrinkled pea seeds (17.4); walnut, potato, smooth pea and pear gave values from 12.6 to 11.0, common bean and broad bean - 10.3 and 9.7, Triticale, carrot, rye, wheat and garden parsley from 8.7 to 8.0 and maize, oat, normal rice from 7.6 to 6.2. The B.V. for amylose was 25.3, for potato starch 12.4, soluble starch 11.9, wheat starch 8.8 and for Triticale and rye starches, 8.7.
topic Blue Value
starch determination
starch-iodine complex
starch in plant materials
starch dissolution in CaCl2
url https://pbsociety.org.pl/journals/index.php/asbp/article/view/1141
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