Label-free characterization of single extracellular vesicles using two-photon fluorescence lifetime imaging microscopy of NAD(P)H

Abstract The heterogeneous nature of extracellular vesicles (EVs) creates the need for single EV characterization techniques. However, many common biochemical and functional EV analysis techniques lack single EV resolution. Two-photon fluorescence lifetime imaging microscopy (FLIM) is widely used to...

Full description

Bibliographic Details
Main Authors: Janet E. Sorrells, Elisabeth M. Martin, Edita Aksamitiene, Prabuddha Mukherjee, Aneesh Alex, Eric J. Chaney, Marina Marjanovic, Stephen A. Boppart
Format: Article
Language:English
Published: Nature Publishing Group 2021-02-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-020-80813-0