Kajian Formulasi Bacillus thuringiensis Dengan Carrier Limbah Cair Pabrik Kelapa Sawit Untuk Pengendalian Ulat Api (Setora nitens)

• Main Author: Dwi Wahyuono
• Format: Article
• Language: English
• Published: Universitas Muhammadiyah Yogyakarta 2015-02-01
• Series: Planta Tropika: Jurnal Agrosains
• Subjects: alternative media; bacillus thuringiensis; bioassay
• Online Access: https://journal.umy.ac.id/index.php/pt/article/view/2520
• ISSN: 0216-499X; 2528-7079

<p>This research aims to examine palm oil effluent as a carrier for <em>B. thuringiensis</em> and its effectiveness against <em>Setora nitens</em> instar II-III in palm plantation. This research was held on April – December 2013 using single factor experiment method that has been arranging in completely randomized design (CRD). The research have two stages, first stage: development of <em>B</em><em> .thuringiensis</em> and second stage: testing bioassay. First stage consist of three treatments that is 100%, 75%, 50% of Palm Oil Effluent and Luria Bertani Broth as a possitive control. Every treatment was added 0,4 g red sugar and 30 ml coconut water. the treatment in second stage same with first stage but use destilled water in addition treatment.  The parameter used are viability of <em>B. thuringiensis</em>, mortality (%), the speed of mortality, the change of population (%), and anti feedant (%). The result shown that palm oil effluent serve the purpose of alternative media of development <em>B. Thuringiensis</em> 7,6 x 10⁷ CFU/ml. The optimal incubation period based of the toxcicity that is 48 hours. Formulation LCPKS 100% + 0,4 g red sugar + 30 ml coconut water + <em>B. thuringiensis</em> inclined more better based on the parameter of mortality, the change of population precentage, the speed of mortality amd anti feedant.</p>