Protective effects of triptolide on retinal ganglion cells in a rat model of chronic glaucoma

Fan Yang, Dongmei Wang, Lingling Wu, Ying Li Ophthalmology Department, Peking University Third Hospital, Beijing, People’s Republic of China Purpose: To study the effects of triptolide, a Chinese herb extract, on retinal ganglion cells (RGCs) in a rat model of chronic glaucoma.Methods: E...

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Main Authors: Yang F, Wang DM, Wu LL, Li Y
Format: Article
Language:English
Published: Dove Medical Press 2015-11-01
Series:Drug Design, Development and Therapy
Online Access:https://www.dovepress.com/protective-effects-of-triptolide-on-retinal-ganglion-cells-in-a-rat-mo-peer-reviewed-article-DDDT
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spelling doaj-4b78ef5c8dbc4b3990111d70d4de34f62020-11-24T20:54:55ZengDove Medical PressDrug Design, Development and Therapy1177-88812015-11-012015default6095610724636Protective effects of triptolide on retinal ganglion cells in a rat model of chronic glaucomaYang FWang DMWu LLLi YFan Yang, Dongmei Wang, Lingling Wu, Ying Li Ophthalmology Department, Peking University Third Hospital, Beijing, People’s Republic of China Purpose: To study the effects of triptolide, a Chinese herb extract, on retinal ganglion cells (RGCs) in a rat model of chronic glaucoma.Methods: Eighty Wistar rats were randomly divided into triptolide group (n=40) and normal saline (NS) group (n=40). Angle photocoagulation was used to establish the model of glaucoma, with right eye as laser treated eye and left eye as control eye. Triptolide group received triptolide intraperitoneally daily, while NS group received NS. Intraocular pressure (IOP), anti-CD11b immunofluorescent stain in retina and optic nerve, RGCs count with Nissel stain and microglia count with anti-CD11b immunofluorescence stain in retina flat mounts, retinal tumor necrosis factor (TNF)-α mRNA detection by reverse transcription–polymerase chain reaction, and double immunofluorescent labeling with anti-TNF-α and anti-CD11b in retinal frozen section were performed.Results: Mean IOP of the laser treated eyes significantly increased 3 weeks after photocoagulation (P<0.05), with no statistical difference between the two groups (P>0.05). RGCs survival in the laser treated eyes was significantly improved in the triptolide group than the NS group (P<0.05). Microglia count in superficial retina of the laser treated eyes was significantly less in the triptolide group (30.40±4.90) than the NS group (35.06±7.59) (P<0.05). TNF-α mRNA expression in the retina of the laser treated eyes in the triptolide group decreased by 60% compared with that in the NS group (P<0.01). The double immunofluorescent labeling showed that TNF-α was mainly distributed around the microglia.Conclusion: Triptolide improved RGCs survival in this rat model of chronic glaucoma, which did not depend on IOP decrease but might be exerted by inhibiting microglia activities and reducing TNF-α secretion. Keywords: glaucoma, triptolide, microglia, neuroprotectionhttps://www.dovepress.com/protective-effects-of-triptolide-on-retinal-ganglion-cells-in-a-rat-mo-peer-reviewed-article-DDDT
collection DOAJ
language English
format Article
sources DOAJ
author Yang F
Wang DM
Wu LL
Li Y
spellingShingle Yang F
Wang DM
Wu LL
Li Y
Protective effects of triptolide on retinal ganglion cells in a rat model of chronic glaucoma
Drug Design, Development and Therapy
author_facet Yang F
Wang DM
Wu LL
Li Y
author_sort Yang F
title Protective effects of triptolide on retinal ganglion cells in a rat model of chronic glaucoma
title_short Protective effects of triptolide on retinal ganglion cells in a rat model of chronic glaucoma
title_full Protective effects of triptolide on retinal ganglion cells in a rat model of chronic glaucoma
title_fullStr Protective effects of triptolide on retinal ganglion cells in a rat model of chronic glaucoma
title_full_unstemmed Protective effects of triptolide on retinal ganglion cells in a rat model of chronic glaucoma
title_sort protective effects of triptolide on retinal ganglion cells in a rat model of chronic glaucoma
publisher Dove Medical Press
series Drug Design, Development and Therapy
issn 1177-8881
publishDate 2015-11-01
description Fan Yang, Dongmei Wang, Lingling Wu, Ying Li Ophthalmology Department, Peking University Third Hospital, Beijing, People’s Republic of China Purpose: To study the effects of triptolide, a Chinese herb extract, on retinal ganglion cells (RGCs) in a rat model of chronic glaucoma.Methods: Eighty Wistar rats were randomly divided into triptolide group (n=40) and normal saline (NS) group (n=40). Angle photocoagulation was used to establish the model of glaucoma, with right eye as laser treated eye and left eye as control eye. Triptolide group received triptolide intraperitoneally daily, while NS group received NS. Intraocular pressure (IOP), anti-CD11b immunofluorescent stain in retina and optic nerve, RGCs count with Nissel stain and microglia count with anti-CD11b immunofluorescence stain in retina flat mounts, retinal tumor necrosis factor (TNF)-α mRNA detection by reverse transcription–polymerase chain reaction, and double immunofluorescent labeling with anti-TNF-α and anti-CD11b in retinal frozen section were performed.Results: Mean IOP of the laser treated eyes significantly increased 3 weeks after photocoagulation (P<0.05), with no statistical difference between the two groups (P>0.05). RGCs survival in the laser treated eyes was significantly improved in the triptolide group than the NS group (P<0.05). Microglia count in superficial retina of the laser treated eyes was significantly less in the triptolide group (30.40±4.90) than the NS group (35.06±7.59) (P<0.05). TNF-α mRNA expression in the retina of the laser treated eyes in the triptolide group decreased by 60% compared with that in the NS group (P<0.01). The double immunofluorescent labeling showed that TNF-α was mainly distributed around the microglia.Conclusion: Triptolide improved RGCs survival in this rat model of chronic glaucoma, which did not depend on IOP decrease but might be exerted by inhibiting microglia activities and reducing TNF-α secretion. Keywords: glaucoma, triptolide, microglia, neuroprotection
url https://www.dovepress.com/protective-effects-of-triptolide-on-retinal-ganglion-cells-in-a-rat-mo-peer-reviewed-article-DDDT
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