Editing DNA Methylation in Mammalian Embryos

Editing DNA Methylation in Mammalian Embryos

DNA methylation in mammals is essential for numerous biological functions, such as ensuring chromosomal stability, genomic imprinting, and X-chromosome inactivation through transcriptional regulation. Gene knockout of DNA methyltransferases and demethylation enzymes has made significant contribution...

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Bibliographic Details
Main Authors: Taiga Yamazaki, Yu Hatano, Ryoya Taniguchi, Noritada Kobayashi, Kazuo Yamagata
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:International Journal of Molecular Sciences
Subjects:
dna methylation
epigenome editing
preimplantation embryo
germ cell
centromere
pericentromere
Online Access:https://www.mdpi.com/1422-0067/21/2/637
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spelling doaj-4b7291ebcaf340f3b1536da7b6d9fd142020-11-25T01:38:06ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-01-0121263710.3390/ijms21020637ijms21020637Editing DNA Methylation in Mammalian EmbryosTaiga Yamazaki0Yu Hatano1Ryoya Taniguchi2Noritada Kobayashi3Kazuo Yamagata4Division of Biomedical Research, Kitasato University Medical Center, Kitasato University, 6-100 Arai, Kitamoto, Saitama 364-8501, JapanFaculty of Biology-Oriented Science and Technology, KINDAI University, 930 Nishimitani, Kinokawa, Wakayama 649-6493, JapanFaculty of Biology-Oriented Science and Technology, KINDAI University, 930 Nishimitani, Kinokawa, Wakayama 649-6493, JapanDivision of Biomedical Research, Kitasato University Medical Center, Kitasato University, 6-100 Arai, Kitamoto, Saitama 364-8501, JapanFaculty of Biology-Oriented Science and Technology, KINDAI University, 930 Nishimitani, Kinokawa, Wakayama 649-6493, JapanDNA methylation in mammals is essential for numerous biological functions, such as ensuring chromosomal stability, genomic imprinting, and X-chromosome inactivation through transcriptional regulation. Gene knockout of DNA methyltransferases and demethylation enzymes has made significant contributions to analyzing the functions of DNA methylation in development. By applying epigenome editing, it is now possible to manipulate DNA methylation in specific genomic regions and to understand the functions of these modifications. In this review, we first describe recent DNA methylation editing technology. We then focused on changes in DNA methylation status during mammalian gametogenesis and preimplantation development, and have discussed the implications of applying this technology to early embryos.https://www.mdpi.com/1422-0067/21/2/637dna methylationepigenome editingpreimplantation embryogerm cellcentromerepericentromere
collection DOAJ
language English
format Article
sources DOAJ
author Taiga Yamazaki
Yu Hatano
Ryoya Taniguchi
Noritada Kobayashi
Kazuo Yamagata
spellingShingle Taiga Yamazaki
Yu Hatano
Ryoya Taniguchi
Noritada Kobayashi
Kazuo Yamagata
Editing DNA Methylation in Mammalian Embryos
International Journal of Molecular Sciences
dna methylation
epigenome editing
preimplantation embryo
germ cell
centromere
pericentromere
author_facet Taiga Yamazaki
Yu Hatano
Ryoya Taniguchi
Noritada Kobayashi
Kazuo Yamagata
author_sort Taiga Yamazaki
title Editing DNA Methylation in Mammalian Embryos
title_short Editing DNA Methylation in Mammalian Embryos
title_full Editing DNA Methylation in Mammalian Embryos
title_fullStr Editing DNA Methylation in Mammalian Embryos
title_full_unstemmed Editing DNA Methylation in Mammalian Embryos
title_sort editing dna methylation in mammalian embryos
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2020-01-01
description DNA methylation in mammals is essential for numerous biological functions, such as ensuring chromosomal stability, genomic imprinting, and X-chromosome inactivation through transcriptional regulation. Gene knockout of DNA methyltransferases and demethylation enzymes has made significant contributions to analyzing the functions of DNA methylation in development. By applying epigenome editing, it is now possible to manipulate DNA methylation in specific genomic regions and to understand the functions of these modifications. In this review, we first describe recent DNA methylation editing technology. We then focused on changes in DNA methylation status during mammalian gametogenesis and preimplantation development, and have discussed the implications of applying this technology to early embryos.
topic dna methylation
epigenome editing
preimplantation embryo
germ cell
centromere
pericentromere
url https://www.mdpi.com/1422-0067/21/2/637
work_keys_str_mv AT taigayamazaki editingdnamethylationinmammalianembryos
AT yuhatano editingdnamethylationinmammalianembryos
AT ryoyataniguchi editingdnamethylationinmammalianembryos
AT noritadakobayashi editingdnamethylationinmammalianembryos
AT kazuoyamagata editingdnamethylationinmammalianembryos
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