Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological Activities

The major groups of antioxidant compounds (isoflavonoids, xanthones, hydroxycinnamic acids) in the rhizome methanol extracts of four Ukrainian <i>Iris</i> sp. (<i>Iris pallida</i>, <i>Iris hungarica</i>, <i>Iris sibirica</i>, and <i>Iris variegat...

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Main Authors: Olha Mykhailenko, Michal Korinek, Liudas Ivanauskas, Ivan Bezruk, Artem Myhal, Vilma Petrikaitė, Mohamed El-Shazly, Guan-Hua Lin, Chia-Yi Lin, Chia-Hung Yen, Bing-Hung Chen, Victoriya Georgiyants, Tsong-Long Hwang
Format: Article
Language:English
Published: MDPI AG 2020-10-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/25/19/4588
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spelling doaj-4b47a3581ddb417e81f31434fced9d522020-11-25T01:54:17ZengMDPI AGMolecules1420-30492020-10-01254588458810.3390/molecules25194588Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological ActivitiesOlha Mykhailenko0Michal Korinek1Liudas Ivanauskas2Ivan Bezruk3Artem Myhal4Vilma Petrikaitė5Mohamed El-Shazly6Guan-Hua Lin7Chia-Yi Lin8Chia-Hung Yen9Bing-Hung Chen10Victoriya Georgiyants11Tsong-Long Hwang12Department of Pharmaceutical Chemistry, National University of Pharmacy, 4-Valentinivska st., 61168 Kharkiv, UkraineDepartment of Biotechnology, College of Life Science, Kaohsiung Medical University, Kaohsiung 80708, TaiwanDepartment of Analytical and Toxicological Chemistry, Lithuanian University of Health Sciences, A. Mickevičiaus g. 9, LT 44307 Kaunas, LithuaniaDepartment of Pharmaceutical Chemistry, National University of Pharmacy, 4-Valentinivska st., 61168 Kharkiv, UkraineDepartment of Pharmaceutical Chemistry, National University of Pharmacy, 4-Valentinivska st., 61168 Kharkiv, UkraineLaboratory of Drug Targets Histopathology, Institute of Cardiology, Lithuanian University of Health Sciences, Sukilėlių pr. 13, LT-50162 Kaunas, LithuaniaDepartment of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, the German University in Cairo, Cairo 11835, EgyptDepartment of Biochemistry and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan 33302, TaiwanDepartment of Biochemistry and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan 33302, TaiwanGraduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, TaiwanDepartment of Biotechnology, College of Life Science, Kaohsiung Medical University, Kaohsiung 80708, TaiwanDepartment of Pharmaceutical Chemistry, National University of Pharmacy, 4-Valentinivska st., 61168 Kharkiv, UkraineGraduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 33302, TaiwanThe major groups of antioxidant compounds (isoflavonoids, xanthones, hydroxycinnamic acids) in the rhizome methanol extracts of four Ukrainian <i>Iris</i> sp. (<i>Iris pallida</i>, <i>Iris hungarica</i>, <i>Iris sibirica</i>, and <i>Iris variegata</i>) were qualitatively and quantitatively analyzed using HPLC-DAD and UPLC-MS/MS. Gallic acid, caffeic acid, mangiferin, tectoridin, irigenin, iristectorigenin B, irisolidone, 5,6-dihydroxy-7,8,3′,5′-tetramethoxyisoflavone, irisolidone-7-<i>O</i>-<i>β</i>-d-glucopyranoside, germanaism B, and nigricin were recognized by comparing their UV/MS spectra, chromatographic retention time (tR) with those of standard reference compounds. <i>I. hungarica</i> and <i>I. variegata</i> showed the highest total amount of phenolic compounds. Germanaism B was the most abundant component in the rhizomes of <i>I. variegata</i> (7.089 ± 0.032 mg/g) and <i>I. hungarica</i> (6.285 ± 0.030 mg/g). The compound analyses showed good calibration curve linearity (r<sup>2</sup> > 0.999) and low detection and quantifications limit. These results validated the method for its use in the simultaneous quantitative evaluation of phenolic compounds in the studied <i>Iris</i> sp. <i>I. hungarica</i> and <i>I. variegata</i> rhizomes exhibited antioxidant activity, as demonstrated by the HPLC-ABTS system and NRF2 expression assay and anti-inflammatory activity on respiratory burst in human neutrophils. Moreover, the extracts showed anti-allergic and cytotoxic effects against cancer cells. Anti-coronavirus 229E and lipid formation activities were also evaluated. In summary, potent antioxidant marker compounds were identified in the examined <i>Iris</i> sp.https://www.mdpi.com/1420-3049/25/19/4588<i>Iris</i> rhizomesHPLC-DADUPLC–MS/MSphenolic compoundsHPLC-ABTSantioxidant
collection DOAJ
language English
format Article
sources DOAJ
author Olha Mykhailenko
Michal Korinek
Liudas Ivanauskas
Ivan Bezruk
Artem Myhal
Vilma Petrikaitė
Mohamed El-Shazly
Guan-Hua Lin
Chia-Yi Lin
Chia-Hung Yen
Bing-Hung Chen
Victoriya Georgiyants
Tsong-Long Hwang
spellingShingle Olha Mykhailenko
Michal Korinek
Liudas Ivanauskas
Ivan Bezruk
Artem Myhal
Vilma Petrikaitė
Mohamed El-Shazly
Guan-Hua Lin
Chia-Yi Lin
Chia-Hung Yen
Bing-Hung Chen
Victoriya Georgiyants
Tsong-Long Hwang
Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological Activities
Molecules
<i>Iris</i> rhizomes
HPLC-DAD
UPLC–MS/MS
phenolic compounds
HPLC-ABTS
antioxidant
author_facet Olha Mykhailenko
Michal Korinek
Liudas Ivanauskas
Ivan Bezruk
Artem Myhal
Vilma Petrikaitė
Mohamed El-Shazly
Guan-Hua Lin
Chia-Yi Lin
Chia-Hung Yen
Bing-Hung Chen
Victoriya Georgiyants
Tsong-Long Hwang
author_sort Olha Mykhailenko
title Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological Activities
title_short Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological Activities
title_full Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological Activities
title_fullStr Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological Activities
title_full_unstemmed Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological Activities
title_sort qualitative and quantitative analysis of ukrainian <i>iris</i> species: a fresh look on their antioxidant content and biological activities
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2020-10-01
description The major groups of antioxidant compounds (isoflavonoids, xanthones, hydroxycinnamic acids) in the rhizome methanol extracts of four Ukrainian <i>Iris</i> sp. (<i>Iris pallida</i>, <i>Iris hungarica</i>, <i>Iris sibirica</i>, and <i>Iris variegata</i>) were qualitatively and quantitatively analyzed using HPLC-DAD and UPLC-MS/MS. Gallic acid, caffeic acid, mangiferin, tectoridin, irigenin, iristectorigenin B, irisolidone, 5,6-dihydroxy-7,8,3′,5′-tetramethoxyisoflavone, irisolidone-7-<i>O</i>-<i>β</i>-d-glucopyranoside, germanaism B, and nigricin were recognized by comparing their UV/MS spectra, chromatographic retention time (tR) with those of standard reference compounds. <i>I. hungarica</i> and <i>I. variegata</i> showed the highest total amount of phenolic compounds. Germanaism B was the most abundant component in the rhizomes of <i>I. variegata</i> (7.089 ± 0.032 mg/g) and <i>I. hungarica</i> (6.285 ± 0.030 mg/g). The compound analyses showed good calibration curve linearity (r<sup>2</sup> > 0.999) and low detection and quantifications limit. These results validated the method for its use in the simultaneous quantitative evaluation of phenolic compounds in the studied <i>Iris</i> sp. <i>I. hungarica</i> and <i>I. variegata</i> rhizomes exhibited antioxidant activity, as demonstrated by the HPLC-ABTS system and NRF2 expression assay and anti-inflammatory activity on respiratory burst in human neutrophils. Moreover, the extracts showed anti-allergic and cytotoxic effects against cancer cells. Anti-coronavirus 229E and lipid formation activities were also evaluated. In summary, potent antioxidant marker compounds were identified in the examined <i>Iris</i> sp.
topic <i>Iris</i> rhizomes
HPLC-DAD
UPLC–MS/MS
phenolic compounds
HPLC-ABTS
antioxidant
url https://www.mdpi.com/1420-3049/25/19/4588
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