Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological Activities
The major groups of antioxidant compounds (isoflavonoids, xanthones, hydroxycinnamic acids) in the rhizome methanol extracts of four Ukrainian <i>Iris</i> sp. (<i>Iris pallida</i>, <i>Iris hungarica</i>, <i>Iris sibirica</i>, and <i>Iris variegat...
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doaj-4b47a3581ddb417e81f31434fced9d522020-11-25T01:54:17ZengMDPI AGMolecules1420-30492020-10-01254588458810.3390/molecules25194588Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological ActivitiesOlha Mykhailenko0Michal Korinek1Liudas Ivanauskas2Ivan Bezruk3Artem Myhal4Vilma Petrikaitė5Mohamed El-Shazly6Guan-Hua Lin7Chia-Yi Lin8Chia-Hung Yen9Bing-Hung Chen10Victoriya Georgiyants11Tsong-Long Hwang12Department of Pharmaceutical Chemistry, National University of Pharmacy, 4-Valentinivska st., 61168 Kharkiv, UkraineDepartment of Biotechnology, College of Life Science, Kaohsiung Medical University, Kaohsiung 80708, TaiwanDepartment of Analytical and Toxicological Chemistry, Lithuanian University of Health Sciences, A. Mickevičiaus g. 9, LT 44307 Kaunas, LithuaniaDepartment of Pharmaceutical Chemistry, National University of Pharmacy, 4-Valentinivska st., 61168 Kharkiv, UkraineDepartment of Pharmaceutical Chemistry, National University of Pharmacy, 4-Valentinivska st., 61168 Kharkiv, UkraineLaboratory of Drug Targets Histopathology, Institute of Cardiology, Lithuanian University of Health Sciences, Sukilėlių pr. 13, LT-50162 Kaunas, LithuaniaDepartment of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, the German University in Cairo, Cairo 11835, EgyptDepartment of Biochemistry and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan 33302, TaiwanDepartment of Biochemistry and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan 33302, TaiwanGraduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, TaiwanDepartment of Biotechnology, College of Life Science, Kaohsiung Medical University, Kaohsiung 80708, TaiwanDepartment of Pharmaceutical Chemistry, National University of Pharmacy, 4-Valentinivska st., 61168 Kharkiv, UkraineGraduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 33302, TaiwanThe major groups of antioxidant compounds (isoflavonoids, xanthones, hydroxycinnamic acids) in the rhizome methanol extracts of four Ukrainian <i>Iris</i> sp. (<i>Iris pallida</i>, <i>Iris hungarica</i>, <i>Iris sibirica</i>, and <i>Iris variegata</i>) were qualitatively and quantitatively analyzed using HPLC-DAD and UPLC-MS/MS. Gallic acid, caffeic acid, mangiferin, tectoridin, irigenin, iristectorigenin B, irisolidone, 5,6-dihydroxy-7,8,3′,5′-tetramethoxyisoflavone, irisolidone-7-<i>O</i>-<i>β</i>-d-glucopyranoside, germanaism B, and nigricin were recognized by comparing their UV/MS spectra, chromatographic retention time (tR) with those of standard reference compounds. <i>I. hungarica</i> and <i>I. variegata</i> showed the highest total amount of phenolic compounds. Germanaism B was the most abundant component in the rhizomes of <i>I. variegata</i> (7.089 ± 0.032 mg/g) and <i>I. hungarica</i> (6.285 ± 0.030 mg/g). The compound analyses showed good calibration curve linearity (r<sup>2</sup> > 0.999) and low detection and quantifications limit. These results validated the method for its use in the simultaneous quantitative evaluation of phenolic compounds in the studied <i>Iris</i> sp. <i>I. hungarica</i> and <i>I. variegata</i> rhizomes exhibited antioxidant activity, as demonstrated by the HPLC-ABTS system and NRF2 expression assay and anti-inflammatory activity on respiratory burst in human neutrophils. Moreover, the extracts showed anti-allergic and cytotoxic effects against cancer cells. Anti-coronavirus 229E and lipid formation activities were also evaluated. In summary, potent antioxidant marker compounds were identified in the examined <i>Iris</i> sp.https://www.mdpi.com/1420-3049/25/19/4588<i>Iris</i> rhizomesHPLC-DADUPLC–MS/MSphenolic compoundsHPLC-ABTSantioxidant |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Olha Mykhailenko Michal Korinek Liudas Ivanauskas Ivan Bezruk Artem Myhal Vilma Petrikaitė Mohamed El-Shazly Guan-Hua Lin Chia-Yi Lin Chia-Hung Yen Bing-Hung Chen Victoriya Georgiyants Tsong-Long Hwang |
spellingShingle |
Olha Mykhailenko Michal Korinek Liudas Ivanauskas Ivan Bezruk Artem Myhal Vilma Petrikaitė Mohamed El-Shazly Guan-Hua Lin Chia-Yi Lin Chia-Hung Yen Bing-Hung Chen Victoriya Georgiyants Tsong-Long Hwang Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological Activities Molecules <i>Iris</i> rhizomes HPLC-DAD UPLC–MS/MS phenolic compounds HPLC-ABTS antioxidant |
author_facet |
Olha Mykhailenko Michal Korinek Liudas Ivanauskas Ivan Bezruk Artem Myhal Vilma Petrikaitė Mohamed El-Shazly Guan-Hua Lin Chia-Yi Lin Chia-Hung Yen Bing-Hung Chen Victoriya Georgiyants Tsong-Long Hwang |
author_sort |
Olha Mykhailenko |
title |
Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological Activities |
title_short |
Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological Activities |
title_full |
Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological Activities |
title_fullStr |
Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological Activities |
title_full_unstemmed |
Qualitative and Quantitative Analysis of Ukrainian <i>Iris</i> Species: A Fresh Look on Their Antioxidant Content and Biological Activities |
title_sort |
qualitative and quantitative analysis of ukrainian <i>iris</i> species: a fresh look on their antioxidant content and biological activities |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-10-01 |
description |
The major groups of antioxidant compounds (isoflavonoids, xanthones, hydroxycinnamic acids) in the rhizome methanol extracts of four Ukrainian <i>Iris</i> sp. (<i>Iris pallida</i>, <i>Iris hungarica</i>, <i>Iris sibirica</i>, and <i>Iris variegata</i>) were qualitatively and quantitatively analyzed using HPLC-DAD and UPLC-MS/MS. Gallic acid, caffeic acid, mangiferin, tectoridin, irigenin, iristectorigenin B, irisolidone, 5,6-dihydroxy-7,8,3′,5′-tetramethoxyisoflavone, irisolidone-7-<i>O</i>-<i>β</i>-d-glucopyranoside, germanaism B, and nigricin were recognized by comparing their UV/MS spectra, chromatographic retention time (tR) with those of standard reference compounds. <i>I. hungarica</i> and <i>I. variegata</i> showed the highest total amount of phenolic compounds. Germanaism B was the most abundant component in the rhizomes of <i>I. variegata</i> (7.089 ± 0.032 mg/g) and <i>I. hungarica</i> (6.285 ± 0.030 mg/g). The compound analyses showed good calibration curve linearity (r<sup>2</sup> > 0.999) and low detection and quantifications limit. These results validated the method for its use in the simultaneous quantitative evaluation of phenolic compounds in the studied <i>Iris</i> sp. <i>I. hungarica</i> and <i>I. variegata</i> rhizomes exhibited antioxidant activity, as demonstrated by the HPLC-ABTS system and NRF2 expression assay and anti-inflammatory activity on respiratory burst in human neutrophils. Moreover, the extracts showed anti-allergic and cytotoxic effects against cancer cells. Anti-coronavirus 229E and lipid formation activities were also evaluated. In summary, potent antioxidant marker compounds were identified in the examined <i>Iris</i> sp. |
topic |
<i>Iris</i> rhizomes HPLC-DAD UPLC–MS/MS phenolic compounds HPLC-ABTS antioxidant |
url |
https://www.mdpi.com/1420-3049/25/19/4588 |
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