Evidence of a New MoYpd1p Phosphotransferase Isoform in the Multistep Phosphorelay System of <i>Magnaporthe oryzae</i>

• Main Authors: Sri Bühring, Alexander Yemelin, Thomas Michna, Stefan Tenzer, Stefan Jacob
• Format: Article
• Language: English
• Published: MDPI AG 2021-05-01
• Series: Journal of Fungi
• Subjects: phosphorelay; high osmolarity glycerol (HOG) pathway; alternative splicing; signaling; histidine kinases; <i>Magnaporthe oryzae</i>
• Online Access: https://www.mdpi.com/2309-608X/7/5/389

Different external stimuli are perceived by multiple sensor histidine kinases and transmitted by phosphorylation via the phosphotransfer protein Ypd1p in the multistep phosphorelay system of the high osmolarity glycerol signaling pathway of filamentous fungi. How the signal propagation takes place is still not known in detail since multiple sensor histidine kinase genes in most filamentous fungi are coded in the genome, whereas only one gene for Ypd1p exists. That raises the hypothesis that various Ypd1p isoforms are produced from a single gene sequence, perhaps by alternative splicing, facilitating a higher variability in signal transduction. We found that the mRNA of <i>MoYPD1</i> in the rice blast fungus <i>Magnaporthe oryzae</i> is subjected to an increased structural variation and amplified putative isoforms on a cDNA level. We then generated mutant strains overexpressing these isoforms, purified the products, and present here one previously unknown MoYpd1p isoform on a proteome level. Alternative splicing was found to be a valid molecular mechanism to increase the signal diversity in eukaryotic multistep phosphorelay systems.