A Gold Nanoparticles Enhanced Surface Plasmon Resonance Immunosensor for Highly Sensitive Detection of Ischemia-Modified Albumin

• Main Authors: Guang Li, Xian Li, Meng Yang, Meng-Meng Chen, Long-Cong Chen, Xing-Liang Xiong
• Format: Article
• Language: English
• Published: MDPI AG 2013-09-01
• Series: Sensors
• Subjects: ischemia modified albumin; surface plasmon resonance immunosensor; gold nanoparticles; signal enhancement
• Online Access: http://www.mdpi.com/1424-8220/13/10/12794

In this study a novel sensitive nanogold particle sensor enhancement based on mixed self-assembled monolayers was explored and used to construct a Surface Plasmon Resonance (SPR) immunosensor to detect Ischemia Modified Albumin (IMA). Compared with a direct binding SPR assay at a limit of detection (LOD) of 100 ng/L, gold nanoparticles (AuNPs) of 10 nm dramatically improved the LOD of IMA to 10 ng/L. Meanwhile, no interfering substance that may lead to false positive results was identified. These results suggested that the SPR biosensor presented superior properties, and provided a simple label-free strategy to increase assay sensitivity for further acute coronary syndrome (ACS) diagnosis.