Optimization of Ultrasound-Assisted Extraction on Antioxidative Activity of <i>Malus toringoides</i> Using Response Surface Methodology

• Main Authors: Qiang Ye, Li Guo, Hongmei Liu, Yushi Liu, Cunyan Zhang, Cheng Peng, Zhiming Liu, Shan Huang, Bin Li
• Format: Article
• Language: English
• Published: MDPI AG 2019-05-01
• Series: Processes
• Subjects: <i>M.toringoides</i>; human umbilical vein endothelial cells; ultrasonic-assisted extraction; protection rate; response surface methodology
• Online Access: https://www.mdpi.com/2227-9717/7/5/270

Ultrasonic-assisted extraction (UAE) was optimized using response surface methodology (RSM) to maintain the cyto-protective activity of <i>M.toringoides</i> against oxidative stress. The optimal conditions for UAE were a 58 mL/g liquid-solid ratio, a 38 &#176;C extraction temperature, an 85% solvent concentration, and a 19-min extraction time, which resulted in a protection rate of 54.57% against hydrogen peroxide-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). These results were comparable to the predicted value of 53.75%. The extracts showed excellent antioxidant activity, and phlorizin was detected in the dried leaves of <i>Malus.toringoides</i>. The highest yield of phlorizin (101.239 mg/g) was also obtained using these conditions. Taken together, these results showed that the method successfully integrated RSM and partial least squares regression methods to optimize <i>M.toringoides</i> extraction to yield the highest cyto-protective activity and effectively increase the yield of phlorizin from <i>M.toringoides</i>.