Characterization of fluorescent eye markers for mammalian transgenic studies.

Characterization of fluorescent eye markers for mammalian transgenic studies.

Genotyping mice by DNA based methods is both laborious and costly. As an alternative, we systematically examined fluorescent proteins expressed in the lens as transgenic markers for mice. A set of eye markers has been selected such that double and triple transgenic animals can be visually identified...

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Main Authors: Jonathan C Cornett, Sean F Landrette, Tian Xu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3247282?pdf=render
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spelling doaj-4a397e92597949b290c5d07071c9eaa02020-11-25T01:46:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01612e2948610.1371/journal.pone.0029486Characterization of fluorescent eye markers for mammalian transgenic studies.Jonathan C CornettSean F LandretteTian XuGenotyping mice by DNA based methods is both laborious and costly. As an alternative, we systematically examined fluorescent proteins expressed in the lens as transgenic markers for mice. A set of eye markers has been selected such that double and triple transgenic animals can be visually identified and that fluorescence intensity in the eyes can be used to distinguish heterozygous from homozygous mice. Taken together, these eye markers dramatically reduce the time and cost of genotyping transgenics and empower analysis of genetic interaction.http://europepmc.org/articles/PMC3247282?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Jonathan C Cornett
Sean F Landrette
Tian Xu
spellingShingle Jonathan C Cornett
Sean F Landrette
Tian Xu
Characterization of fluorescent eye markers for mammalian transgenic studies.
PLoS ONE
author_facet Jonathan C Cornett
Sean F Landrette
Tian Xu
author_sort Jonathan C Cornett
title Characterization of fluorescent eye markers for mammalian transgenic studies.
title_short Characterization of fluorescent eye markers for mammalian transgenic studies.
title_full Characterization of fluorescent eye markers for mammalian transgenic studies.
title_fullStr Characterization of fluorescent eye markers for mammalian transgenic studies.
title_full_unstemmed Characterization of fluorescent eye markers for mammalian transgenic studies.
title_sort characterization of fluorescent eye markers for mammalian transgenic studies.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2011-01-01
description Genotyping mice by DNA based methods is both laborious and costly. As an alternative, we systematically examined fluorescent proteins expressed in the lens as transgenic markers for mice. A set of eye markers has been selected such that double and triple transgenic animals can be visually identified and that fluorescence intensity in the eyes can be used to distinguish heterozygous from homozygous mice. Taken together, these eye markers dramatically reduce the time and cost of genotyping transgenics and empower analysis of genetic interaction.
url http://europepmc.org/articles/PMC3247282?pdf=render
work_keys_str_mv AT jonathanccornett characterizationoffluorescenteyemarkersformammaliantransgenicstudies
AT seanflandrette characterizationoffluorescenteyemarkersformammaliantransgenicstudies
AT tianxu characterizationoffluorescenteyemarkersformammaliantransgenicstudies
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