Germline transformation of Aedes fluviatilis (Diptera:Culicidae) with the piggyBac transposable element
The technique to generate transgenic mosquitoes requires adaptation for each target species because of aspects related to species biology, sensitivity to manipulation and rearing conditions. Here we tested different parameters on the microinjection procedure in order to obtain a transgenic Neotropic...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Instituto Oswaldo Cruz, Ministério da Saúde
2006-11-01
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| Series: | Memórias do Instituto Oswaldo Cruz. |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000700008 |
| Summary: | The technique to generate transgenic mosquitoes requires adaptation for each target species because of aspects related to species biology, sensitivity to manipulation and rearing conditions. Here we tested different parameters on the microinjection procedure in order to obtain a transgenic Neotropical mosquito species. By using a transposon-based strategy we were able to successfully transform Aedes fluviatilis (Lutz), which can be used as an avian malaria model. These results demonstrate the usefulness of the piggyBac transposable element as a transformation vector for Neotropical mosquito species and opens up new research frontiers for South American mosquito vectors. |
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| ISSN: | 0074-0276 1678-8060 |