Bone density of defects treated with lyophilized amniotic membrane versus colagen membrane: a tomographic and histomorfogenic study in a rabbi´s femur.

• Main Authors: Liz Ríos, Carlos Espinoza, Marco Alarcón, Jorge Huamaní
• Format: Article
• Language: English
• Published: Universidad de Concepción. 2014-09-01
• Series: Journal of Oral Research
• Subjects: amniotic membrane dressings; bone regeneration; Cone-Beam; Dental Implant; biological dressing.
• Online Access: http://www.joralres.com/index.php/JOR/article/view/90/91

The aim of this study was to compare the bone density of bone defects treated with lyophilizated amniotic membrane (LAM) and collagen Membrane (CM), at 3 and 5 weeks. Two bone defects of 4mm in diameter and 6mm deep were created in left distal femoral diaphysis of New Zealand rabbits (n=12). The animals were randomly divided into 2 groups. One of the defects was covered with lyophilized amniotic membrane (Rosa Chambergo Tissue Bank/National Institute of Child Health-IPEN, Lima, Peru) or collagen Membrane (Dentium Co, Seoul, Korea). The second was left uncovered (NC). The rabbits were killed after 3 and 5 weeks (3 rabbits/period). The results showed a high bone density and repair of the defect by new bone. The tomographic study revealed that the bone density of the defects treated with LAM at 3 weeks was equivalent to the density obtained with CM and higher density compared with NC (p<0.05). At 5 weeks, the bone density obtained with LAM was more than density CM and NC (p<0.05). The histomorphometric study showed no significant differences between LAM and CM at 3 and 5 weeks (p>0.05). The results show that lyophilizated amniotic membrane provides bone density equal or higher to the collagen membrane.