High Performance Liquid Chromatographic Analysis of Almotriptan Malate in Bulk and Tablets

• Main Authors: Chandra Bala Sekaran, Chepuri Divya, Petikam lavudu, Avula Prameela Rani
• Format: Article
• Language: English
• Published: Tabriz University of Medical Sciences 2013-02-01
• Series: Advanced Pharmaceutical Bulletin
• Subjects: Almotriptan Malate; HPLC; Validation; Tablets; Analysis
• Online Access: http://journals.tbzmed.ac.ir/PDF/APB/Manuscript/APB-3-183.pdf

Purpose: A simple RP-HPLC method has been developed and validated for the determination of almotriptan malate (ATM) in bulk and tablets. Methods: Chromatographic separation of ATM was achieved by using a Thermo Scientific C18 column. A Mobile phase containing a mixture of methanol, water and acetic acid (4:8:0.1 v/v) was pumped at the flow rate of 1 mL/min. Detection was performed at 227 nm. According to ICH guidelines, the method was validated. Results: The calibration curve was linear in the concentration range 5–60 μg/mL for the ATM with regression coefficient 0.9999. The method was precise with RSD <1.2%. Excellent recoveries of 99.60 - 100.80% proved the accuracy of the method. The limits of detection and quantification were found to be 0.025 and 0.075 μg/mL, respectively. Conclusion: The method was successfully applied for the quantification of ATM in tablets with acceptable accuracy and precision.