Derivation of Huntington Disease affected Genea018 human embryonic stem cell line

Derivation of Huntington Disease affected Genea018 human embryonic stem cell line

The Genea018 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying Htt gene CAG expansion of 46 repeats, indicative of Huntington Disease. Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XX by CGH and STR...

Full description

Bibliographic Details
Main Authors: Biljana Dumevska, Heather Main, Robert McKernan, Divya Goel, Uli Schmidt, Teija Peura
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506116000489
id doaj-48ffee8d16fa41ce9ccd808f1954a62e
record_format Article
spelling doaj-48ffee8d16fa41ce9ccd808f1954a62e2020-11-24T23:06:35ZengElsevierStem Cell Research1873-50611876-77532016-03-0116242342610.1016/j.scr.2016.02.006Derivation of Huntington Disease affected Genea018 human embryonic stem cell lineBiljana DumevskaHeather MainRobert McKernanDivya GoelUli SchmidtTeija PeuraThe Genea018 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying Htt gene CAG expansion of 46 repeats, indicative of Huntington Disease. Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XX by CGH and STR analysis demonstrated a female Allele pattern. The hESC line had pluripotent cell morphology, 75% of cells expressed Nanog, 91% Oct4, 73% Tra1-60 and 96% SSEA4, gave a Pluritest pluripotency score of 31.12, Novelty of 1.45, demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.http://www.sciencedirect.com/science/article/pii/S1873506116000489
collection DOAJ
language English
format Article
sources DOAJ
author Biljana Dumevska
Heather Main
Robert McKernan
Divya Goel
Uli Schmidt
Teija Peura
spellingShingle Biljana Dumevska
Heather Main
Robert McKernan
Divya Goel
Uli Schmidt
Teija Peura
Derivation of Huntington Disease affected Genea018 human embryonic stem cell line
Stem Cell Research
author_facet Biljana Dumevska
Heather Main
Robert McKernan
Divya Goel
Uli Schmidt
Teija Peura
author_sort Biljana Dumevska
title Derivation of Huntington Disease affected Genea018 human embryonic stem cell line
title_short Derivation of Huntington Disease affected Genea018 human embryonic stem cell line
title_full Derivation of Huntington Disease affected Genea018 human embryonic stem cell line
title_fullStr Derivation of Huntington Disease affected Genea018 human embryonic stem cell line
title_full_unstemmed Derivation of Huntington Disease affected Genea018 human embryonic stem cell line
title_sort derivation of huntington disease affected genea018 human embryonic stem cell line
publisher Elsevier
series Stem Cell Research
issn 1873-5061
1876-7753
publishDate 2016-03-01
description The Genea018 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying Htt gene CAG expansion of 46 repeats, indicative of Huntington Disease. Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XX by CGH and STR analysis demonstrated a female Allele pattern. The hESC line had pluripotent cell morphology, 75% of cells expressed Nanog, 91% Oct4, 73% Tra1-60 and 96% SSEA4, gave a Pluritest pluripotency score of 31.12, Novelty of 1.45, demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.
url http://www.sciencedirect.com/science/article/pii/S1873506116000489
work_keys_str_mv AT biljanadumevska derivationofhuntingtondiseaseaffectedgenea018humanembryonicstemcellline
AT heathermain derivationofhuntingtondiseaseaffectedgenea018humanembryonicstemcellline
AT robertmckernan derivationofhuntingtondiseaseaffectedgenea018humanembryonicstemcellline
AT divyagoel derivationofhuntingtondiseaseaffectedgenea018humanembryonicstemcellline
AT ulischmidt derivationofhuntingtondiseaseaffectedgenea018humanembryonicstemcellline
AT teijapeura derivationofhuntingtondiseaseaffectedgenea018humanembryonicstemcellline
_version_ 1725622395120648192

Similar Items