Ciliostasis of airway epithelial cells facilitates influenza A virus infection

Ciliostasis of airway epithelial cells facilitates influenza A virus infection

Abstract Porcine precision-cut lung slices (PCLS) were used to analyze the effect of the ciliary activity on infection of airway epithelial cells by influenza viruses. Treatment of slices with 2% NaCl for 30 min resulted in reversible ciliostasis. When PCLS were infected by a swine influenza virus o...

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Main Authors: Yuguang Fu, Jie Tong, Fandan Meng, Doris Hoeltig, Guangliang Liu, Xiangping Yin, Georg Herrler
Format: Article
Language:English
Published: BMC 2018-07-01
Series:Veterinary Research
Online Access:http://link.springer.com/article/10.1186/s13567-018-0568-0
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spelling doaj-48e90c6e9ed74319b1a68150d8916fb02020-11-25T01:02:28ZengBMCVeterinary Research1297-97162018-07-014911410.1186/s13567-018-0568-0Ciliostasis of airway epithelial cells facilitates influenza A virus infectionYuguang Fu0Jie Tong1Fandan Meng2Doris Hoeltig3Guangliang Liu4Xiangping Yin5Georg Herrler6Institute of Virology, University of Veterinary Medicine Hannover, FoundationInstitute of Virology, University of Veterinary Medicine Hannover, FoundationInstitute of Virology, University of Veterinary Medicine Hannover, FoundationClinic of Swine and Small Ruminants and Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, FoundationLanzhou Veterinary Research Institute, State Key Laboratory of Veterinary Etiological Biology, Chinese Academy of Agricultural SciencesLanzhou Veterinary Research Institute, State Key Laboratory of Veterinary Etiological Biology, Chinese Academy of Agricultural SciencesInstitute of Virology, University of Veterinary Medicine Hannover, FoundationAbstract Porcine precision-cut lung slices (PCLS) were used to analyze the effect of the ciliary activity on infection of airway epithelial cells by influenza viruses. Treatment of slices with 2% NaCl for 30 min resulted in reversible ciliostasis. When PCLS were infected by a swine influenza virus of the H3N2 subtype under ciliostatic conditions, the viral yield was about twofold or threefold higher at 24 or 48 h post-infection, respectively, as compared to slices with ciliary activity. Therefore, the cilia beating not only transports the mucus out of the airways, it also impedes virus infection.http://link.springer.com/article/10.1186/s13567-018-0568-0
collection DOAJ
language English
format Article
sources DOAJ
author Yuguang Fu
Jie Tong
Fandan Meng
Doris Hoeltig
Guangliang Liu
Xiangping Yin
Georg Herrler
spellingShingle Yuguang Fu
Jie Tong
Fandan Meng
Doris Hoeltig
Guangliang Liu
Xiangping Yin
Georg Herrler
Ciliostasis of airway epithelial cells facilitates influenza A virus infection
Veterinary Research
author_facet Yuguang Fu
Jie Tong
Fandan Meng
Doris Hoeltig
Guangliang Liu
Xiangping Yin
Georg Herrler
author_sort Yuguang Fu
title Ciliostasis of airway epithelial cells facilitates influenza A virus infection
title_short Ciliostasis of airway epithelial cells facilitates influenza A virus infection
title_full Ciliostasis of airway epithelial cells facilitates influenza A virus infection
title_fullStr Ciliostasis of airway epithelial cells facilitates influenza A virus infection
title_full_unstemmed Ciliostasis of airway epithelial cells facilitates influenza A virus infection
title_sort ciliostasis of airway epithelial cells facilitates influenza a virus infection
publisher BMC
series Veterinary Research
issn 1297-9716
publishDate 2018-07-01
description Abstract Porcine precision-cut lung slices (PCLS) were used to analyze the effect of the ciliary activity on infection of airway epithelial cells by influenza viruses. Treatment of slices with 2% NaCl for 30 min resulted in reversible ciliostasis. When PCLS were infected by a swine influenza virus of the H3N2 subtype under ciliostatic conditions, the viral yield was about twofold or threefold higher at 24 or 48 h post-infection, respectively, as compared to slices with ciliary activity. Therefore, the cilia beating not only transports the mucus out of the airways, it also impedes virus infection.
url http://link.springer.com/article/10.1186/s13567-018-0568-0
work_keys_str_mv AT yuguangfu ciliostasisofairwayepithelialcellsfacilitatesinfluenzaavirusinfection
AT jietong ciliostasisofairwayepithelialcellsfacilitatesinfluenzaavirusinfection
AT fandanmeng ciliostasisofairwayepithelialcellsfacilitatesinfluenzaavirusinfection
AT dorishoeltig ciliostasisofairwayepithelialcellsfacilitatesinfluenzaavirusinfection
AT guangliangliu ciliostasisofairwayepithelialcellsfacilitatesinfluenzaavirusinfection
AT xiangpingyin ciliostasisofairwayepithelialcellsfacilitatesinfluenzaavirusinfection
AT georgherrler ciliostasisofairwayepithelialcellsfacilitatesinfluenzaavirusinfection
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