A Single Standard to Determine Multi-Components Method Coupled with Chemometric Methods for the Quantification, Evaluation and Classification of <i>Notopterygii</i> Rhizoma et Radix from Different Regions

• Main Authors: Xie-An Yu, Jin Li, John Teye Azietaku, Wei Liu, Jun He, Yan-Xu Chang
• Format: Article
• Language: English
• Published: MDPI AG 2019-10-01
• Series: Molecules
• Subjects: chemometrics; different regions; <i>notopterygii</i> rhizoma and radix
• Online Access: https://www.mdpi.com/1420-3049/24/19/3574

An ultra-high-performance liquid chromatography-quadrupole/time of flight mass spectrometry is used to identify 33 compounds in <i>Notopterygii</i> rhizoma and radix, after which a single standard to determine multi-components method is established for the simultaneous determination of 19 compounds in <i>Notopterygii</i> rhizoma and radix using chlorogenic acid and notopterol as the internal standard. To screen the potential chemical markers among <i>Notopterygii</i> rhizoma and radix planted in its natural germination area and in others, the quantitative data of 19 compounds are analyzed via partial least-squares discriminant analysis (PLS&#8722;DA). Depending on the variable importance parameters (VIP) value of PLS&#8722;DA, six compounds are selected to be the potential chemical markers for the discrimination of <i>Notopterygii</i> rhizoma and radix planted in the different regions. Furthermore, the Fisher&#8217;s discriminant analysis is used to build the models that are used to classify <i>Notopterygii</i> rhizoma and radix from the different regions based on the six chemical markers. Experimental results indicate that <i>Notopterygii</i> rhizoma and radix planted in the Sichuan province are distinguished successfully from those in other regions, reaching a 96.0% accuracy rating. Therefore, a single standard to determine multi-components method combined with a chemometrics method, which contains the advantages such as simple, rapid, economical and accurate identification, offers a new perspective for the quantification, evaluation and classification of <i>Notopterygii</i> rhizoma and radix from the different regions.