| Summary: | An ultra-high-performance liquid chromatography-quadrupole/time of flight mass spectrometry is used to identify 33 compounds in <i>Notopterygii</i> rhizoma and radix, after which a single standard to determine multi-components method is established for the simultaneous determination of 19 compounds in <i>Notopterygii</i> rhizoma and radix using chlorogenic acid and notopterol as the internal standard. To screen the potential chemical markers among <i>Notopterygii</i> rhizoma and radix planted in its natural germination area and in others, the quantitative data of 19 compounds are analyzed via partial least-squares discriminant analysis (PLS−DA). Depending on the variable importance parameters (VIP) value of PLS−DA, six compounds are selected to be the potential chemical markers for the discrimination of <i>Notopterygii</i> rhizoma and radix planted in the different regions. Furthermore, the Fisher’s discriminant analysis is used to build the models that are used to classify <i>Notopterygii</i> rhizoma and radix from the different regions based on the six chemical markers. Experimental results indicate that <i>Notopterygii</i> rhizoma and radix planted in the Sichuan province are distinguished successfully from those in other regions, reaching a 96.0% accuracy rating. Therefore, a single standard to determine multi-components method combined with a chemometrics method, which contains the advantages such as simple, rapid, economical and accurate identification, offers a new perspective for the quantification, evaluation and classification of <i>Notopterygii</i> rhizoma and radix from the different regions.
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