The Effect of Thawing Temperature on Sperm Quality of Friesian Holstein Bulls

The percentage of sperm motility and morphology are important criteria in evaluating the quality of sperm before it is used for artificial insemination (AI). This study was conducted to observe post thawing motility and abnormal morphology of spermatozoa Friesian Holstein (FH). The materials were us...

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Main Authors: Tri Utami, Tarsisius Considus Topianong
Format: Article
Language:Indonesian
Published: Universitas Gadjah Mada 2014-11-01
Series:Jurnal Sain Veteriner
Subjects:
Online Access:https://jurnal.ugm.ac.id/jsv/article/view/5420
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spelling doaj-48be64c4e43947a1be4ee4c95b7b94852020-11-24T22:21:38ZindUniversitas Gadjah MadaJurnal Sain Veteriner0126-04212407-37332014-11-0132110.22146/jsv.54204761The Effect of Thawing Temperature on Sperm Quality of Friesian Holstein BullsTri Utami0Tarsisius Considus Topianong1Bagian Klinik, Reproduksi dan Patologi FKH Universitas Nusa Cendana Kupang NTTBagian Klinik, Reproduksi dan Patologi FKH Universitas Nusa Cendana Kupang NTTThe percentage of sperm motility and morphology are important criteria in evaluating the quality of sperm before it is used for artificial insemination (AI). This study was conducted to observe post thawing motility and abnormal morphology of spermatozoa Friesian Holstein (FH). The materials were used 10 straws of FH bulls in the form of 0.25 ml. A total of 10 straws then divided into two treatment groups of  thawing  in water at 37°C and water 8°C, respectively. An examination of the motility and morphology of spermatozoa abnormalities performed every two hours for two times. Calculating the percentage of sperm motility was done by calculating the percentage of spermatozoa moving forward in the field of view under a microscope with a magnification of 10x. the percentage of abnormal spermatozoa was  assessed by William's stain. Spermatozoa morphology was observed by using a microscope magnification of 100x. Abnormalities of spermatozoa were calculated from a total of 200 spermatozoa, either normal or abnormal. At the same thawing time, the motility of FH cattle sperms post thawing in water temperature 37°C had a higher preference than that of post thawing in water temperature 8°C, although it was not significantly different (P > 0.05).  Based on morphological aspects, frozen semen used in this study is within the tolerance limit for the total percentage of abnormal sperm morphology between 12% to 23% and normal morphology between 70% to 88%.https://jurnal.ugm.ac.id/jsv/article/view/5420Quality of spermatozoa, Friesian Holstein, thawing, morphology, motility
collection DOAJ
language Indonesian
format Article
sources DOAJ
author Tri Utami
Tarsisius Considus Topianong
spellingShingle Tri Utami
Tarsisius Considus Topianong
The Effect of Thawing Temperature on Sperm Quality of Friesian Holstein Bulls
Jurnal Sain Veteriner
Quality of spermatozoa, Friesian Holstein, thawing, morphology, motility
author_facet Tri Utami
Tarsisius Considus Topianong
author_sort Tri Utami
title The Effect of Thawing Temperature on Sperm Quality of Friesian Holstein Bulls
title_short The Effect of Thawing Temperature on Sperm Quality of Friesian Holstein Bulls
title_full The Effect of Thawing Temperature on Sperm Quality of Friesian Holstein Bulls
title_fullStr The Effect of Thawing Temperature on Sperm Quality of Friesian Holstein Bulls
title_full_unstemmed The Effect of Thawing Temperature on Sperm Quality of Friesian Holstein Bulls
title_sort effect of thawing temperature on sperm quality of friesian holstein bulls
publisher Universitas Gadjah Mada
series Jurnal Sain Veteriner
issn 0126-0421
2407-3733
publishDate 2014-11-01
description The percentage of sperm motility and morphology are important criteria in evaluating the quality of sperm before it is used for artificial insemination (AI). This study was conducted to observe post thawing motility and abnormal morphology of spermatozoa Friesian Holstein (FH). The materials were used 10 straws of FH bulls in the form of 0.25 ml. A total of 10 straws then divided into two treatment groups of  thawing  in water at 37°C and water 8°C, respectively. An examination of the motility and morphology of spermatozoa abnormalities performed every two hours for two times. Calculating the percentage of sperm motility was done by calculating the percentage of spermatozoa moving forward in the field of view under a microscope with a magnification of 10x. the percentage of abnormal spermatozoa was  assessed by William's stain. Spermatozoa morphology was observed by using a microscope magnification of 100x. Abnormalities of spermatozoa were calculated from a total of 200 spermatozoa, either normal or abnormal. At the same thawing time, the motility of FH cattle sperms post thawing in water temperature 37°C had a higher preference than that of post thawing in water temperature 8°C, although it was not significantly different (P > 0.05).  Based on morphological aspects, frozen semen used in this study is within the tolerance limit for the total percentage of abnormal sperm morphology between 12% to 23% and normal morphology between 70% to 88%.
topic Quality of spermatozoa, Friesian Holstein, thawing, morphology, motility
url https://jurnal.ugm.ac.id/jsv/article/view/5420
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