Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties
This study was carried out for the downy mildew resistant test between 2010 and 2012. A set of 22 accessions belonging to 2 wild species and 20 varieties of the genus Cucumis, originating mainly from the Asian Vegetable Research and Development Center (AVRDC) Gene Centre, was evaluated for resista...
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Hanrimwon Publishing Company
2013-09-01
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doaj-48b8e84b80594ef1a1c64daeac8c292d2020-11-25T00:13:56ZengHanrimwon Publishing CompanyResearch in Plant Disease1598-22622233-91912013-09-01193188195http://dx.doi.org/10.5423/RPD.2013.19.3.188Screening for Resistance to Downy Mildew among Major Commercial Cucumber VarietiesJung-Sup Lee0Kyung-Sook Han1Seong-Chan Lee2Jae-Woo Soh3Rural Development Administration, Suwon 441-440, KoreaRural Development Administration, Suwon 441-440, KoreaRural Development Administration, Suwon 441-440, KoreaRural Development Administration, Suwon 441-440, KoreaThis study was carried out for the downy mildew resistant test between 2010 and 2012. A set of 22 accessions belonging to 2 wild species and 20 varieties of the genus Cucumis, originating mainly from the Asian Vegetable Research and Development Center (AVRDC) Gene Centre, was evaluated for resistance to Pseudoperonospora cubensis, causal agent of cucumber downy mildew. The youngest fully expanded true leaves were found suitable for in vitro screening. Both leaf discs and full leaves could be kept fresh longer when applying 0.2 μg/ml of gibberellin acid (GA). The incubation temperature of 20oC was found to be the most suitable temperature for symptom development comparing with 15 and 25oC. Symptom development was faster when contact diseased leaf discs (2 weeks after inoculation) on to fresh leaf samples comparing with using conidia suspension (105 spores/ml). The numbers of spots in ‘C-19’ were lower than other varieties. ‘C-19’ variety was also showed the highest level of downy mildew resistant at 20oC chamber in 6 days after inoculating with pathogen and displayed 0.90 (under 10%) of the infected rate. However, other varieties displayed susceptible in the pathogen sprayed plots. ‘C-19’ was the most resistant variety and no lesion was observed. Based on all data, ‘C-19’ can be a useful variety for the prevention of downy mildew.http://www.online-rpd.org/journal/view.html?uid=719&sort=book_Seq&scale=&key=year&keyword=&s_v=19&s_n=3&pn=vol&year=2013&vmd=FullCucumis sativusFruit numberGermplasm evalution |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jung-Sup Lee Kyung-Sook Han Seong-Chan Lee Jae-Woo Soh |
spellingShingle |
Jung-Sup Lee Kyung-Sook Han Seong-Chan Lee Jae-Woo Soh Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties Research in Plant Disease Cucumis sativus Fruit number Germplasm evalution |
author_facet |
Jung-Sup Lee Kyung-Sook Han Seong-Chan Lee Jae-Woo Soh |
author_sort |
Jung-Sup Lee |
title |
Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties |
title_short |
Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties |
title_full |
Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties |
title_fullStr |
Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties |
title_full_unstemmed |
Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties |
title_sort |
screening for resistance to downy mildew among major commercial cucumber varieties |
publisher |
Hanrimwon Publishing Company |
series |
Research in Plant Disease |
issn |
1598-2262 2233-9191 |
publishDate |
2013-09-01 |
description |
This study was carried out for the downy mildew resistant test between 2010 and 2012. A set of 22 accessions
belonging to 2 wild species and 20 varieties of the genus Cucumis, originating mainly from the Asian
Vegetable Research and Development Center (AVRDC) Gene Centre, was evaluated for resistance to
Pseudoperonospora cubensis, causal agent of cucumber downy mildew. The youngest fully expanded true
leaves were found suitable for in vitro screening. Both leaf discs and full leaves could be kept fresh longer
when applying 0.2 μg/ml of gibberellin acid (GA). The incubation temperature of 20oC was found to be the
most suitable temperature for symptom development comparing with 15 and 25oC. Symptom development
was faster when contact diseased leaf discs (2 weeks after inoculation) on to fresh leaf samples comparing
with using conidia suspension (105 spores/ml). The numbers of spots in ‘C-19’ were lower than other varieties.
‘C-19’ variety was also showed the highest level of downy mildew resistant at 20oC chamber in 6 days after
inoculating with pathogen and displayed 0.90 (under 10%) of the infected rate. However, other varieties
displayed susceptible in the pathogen sprayed plots. ‘C-19’ was the most resistant variety and no lesion was
observed. Based on all data, ‘C-19’ can be a useful variety for the prevention of downy mildew. |
topic |
Cucumis sativus Fruit number Germplasm evalution |
url |
http://www.online-rpd.org/journal/view.html?uid=719&sort=book_Seq&scale=&key=year&keyword=&s_v=19&s_n=3&pn=vol&year=2013&vmd=Full |
work_keys_str_mv |
AT jungsuplee screeningforresistancetodownymildewamongmajorcommercialcucumbervarieties AT kyungsookhan screeningforresistancetodownymildewamongmajorcommercialcucumbervarieties AT seongchanlee screeningforresistancetodownymildewamongmajorcommercialcucumbervarieties AT jaewoosoh screeningforresistancetodownymildewamongmajorcommercialcucumbervarieties |
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1725392267721572352 |