Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties

This study was carried out for the downy mildew resistant test between 2010 and 2012. A set of 22 accessions belonging to 2 wild species and 20 varieties of the genus Cucumis, originating mainly from the Asian Vegetable Research and Development Center (AVRDC) Gene Centre, was evaluated for resista...

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Main Authors: Jung-Sup Lee, Kyung-Sook Han, Seong-Chan Lee, Jae-Woo Soh
Format: Article
Language:English
Published: Hanrimwon Publishing Company 2013-09-01
Series:Research in Plant Disease
Subjects:
Online Access:http://www.online-rpd.org/journal/view.html?uid=719&sort=book_Seq&scale=&key=year&keyword=&s_v=19&s_n=3&pn=vol&year=2013&vmd=Full
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spelling doaj-48b8e84b80594ef1a1c64daeac8c292d2020-11-25T00:13:56ZengHanrimwon Publishing CompanyResearch in Plant Disease1598-22622233-91912013-09-01193188195http://dx.doi.org/10.5423/RPD.2013.19.3.188Screening for Resistance to Downy Mildew among Major Commercial Cucumber VarietiesJung-Sup Lee0Kyung-Sook Han1Seong-Chan Lee2Jae-Woo Soh3Rural Development Administration, Suwon 441-440, KoreaRural Development Administration, Suwon 441-440, KoreaRural Development Administration, Suwon 441-440, KoreaRural Development Administration, Suwon 441-440, KoreaThis study was carried out for the downy mildew resistant test between 2010 and 2012. A set of 22 accessions belonging to 2 wild species and 20 varieties of the genus Cucumis, originating mainly from the Asian Vegetable Research and Development Center (AVRDC) Gene Centre, was evaluated for resistance to Pseudoperonospora cubensis, causal agent of cucumber downy mildew. The youngest fully expanded true leaves were found suitable for in vitro screening. Both leaf discs and full leaves could be kept fresh longer when applying 0.2 μg/ml of gibberellin acid (GA). The incubation temperature of 20oC was found to be the most suitable temperature for symptom development comparing with 15 and 25oC. Symptom development was faster when contact diseased leaf discs (2 weeks after inoculation) on to fresh leaf samples comparing with using conidia suspension (105 spores/ml). The numbers of spots in ‘C-19’ were lower than other varieties. ‘C-19’ variety was also showed the highest level of downy mildew resistant at 20oC chamber in 6 days after inoculating with pathogen and displayed 0.90 (under 10%) of the infected rate. However, other varieties displayed susceptible in the pathogen sprayed plots. ‘C-19’ was the most resistant variety and no lesion was observed. Based on all data, ‘C-19’ can be a useful variety for the prevention of downy mildew.http://www.online-rpd.org/journal/view.html?uid=719&sort=book_Seq&scale=&key=year&keyword=&s_v=19&s_n=3&pn=vol&year=2013&vmd=FullCucumis sativusFruit numberGermplasm evalution
collection DOAJ
language English
format Article
sources DOAJ
author Jung-Sup Lee
Kyung-Sook Han
Seong-Chan Lee
Jae-Woo Soh
spellingShingle Jung-Sup Lee
Kyung-Sook Han
Seong-Chan Lee
Jae-Woo Soh
Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties
Research in Plant Disease
Cucumis sativus
Fruit number
Germplasm evalution
author_facet Jung-Sup Lee
Kyung-Sook Han
Seong-Chan Lee
Jae-Woo Soh
author_sort Jung-Sup Lee
title Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties
title_short Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties
title_full Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties
title_fullStr Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties
title_full_unstemmed Screening for Resistance to Downy Mildew among Major Commercial Cucumber Varieties
title_sort screening for resistance to downy mildew among major commercial cucumber varieties
publisher Hanrimwon Publishing Company
series Research in Plant Disease
issn 1598-2262
2233-9191
publishDate 2013-09-01
description This study was carried out for the downy mildew resistant test between 2010 and 2012. A set of 22 accessions belonging to 2 wild species and 20 varieties of the genus Cucumis, originating mainly from the Asian Vegetable Research and Development Center (AVRDC) Gene Centre, was evaluated for resistance to Pseudoperonospora cubensis, causal agent of cucumber downy mildew. The youngest fully expanded true leaves were found suitable for in vitro screening. Both leaf discs and full leaves could be kept fresh longer when applying 0.2 μg/ml of gibberellin acid (GA). The incubation temperature of 20oC was found to be the most suitable temperature for symptom development comparing with 15 and 25oC. Symptom development was faster when contact diseased leaf discs (2 weeks after inoculation) on to fresh leaf samples comparing with using conidia suspension (105 spores/ml). The numbers of spots in ‘C-19’ were lower than other varieties. ‘C-19’ variety was also showed the highest level of downy mildew resistant at 20oC chamber in 6 days after inoculating with pathogen and displayed 0.90 (under 10%) of the infected rate. However, other varieties displayed susceptible in the pathogen sprayed plots. ‘C-19’ was the most resistant variety and no lesion was observed. Based on all data, ‘C-19’ can be a useful variety for the prevention of downy mildew.
topic Cucumis sativus
Fruit number
Germplasm evalution
url http://www.online-rpd.org/journal/view.html?uid=719&sort=book_Seq&scale=&key=year&keyword=&s_v=19&s_n=3&pn=vol&year=2013&vmd=Full
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