Monitoring of α-amylase activity with commercial digital multimeter

This work presents the construction of a potentiometric system for simple monitoring of enzymatic activity of alpha-amylase using a commom multimeter together with its application em laboratory classes ( higher education or basic education). The results presented here points to 1) novelty and potent...

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Bibliographic Details
Main Authors: George Augusto Veloso de Oliveira, José Maurício Shennedorf Ferreira da Silva
Format: Article
Language:Portuguese
Published: Sociedade Brasileira de Bioquímica e Biologia Molecular, SBBq 2019-06-01
Series:Journal of Biochemistry Education
Subjects:
Online Access:http://bioquimica.org.br/revista/ojs/index.php/REB/article/view/855
Description
Summary:This work presents the construction of a potentiometric system for simple monitoring of enzymatic activity of alpha-amylase using a commom multimeter together with its application em laboratory classes ( higher education or basic education). The results presented here points to 1) novelty and potential method for applying a quantitative teaching about enzymatic kinetics in Biochemistry classes, 2) practical classes with minimal resources and reagents found in local commerce, as well as 3) the facilitation of the concepts learning and data analysis in enzymatic kinetics. In the experiment, a coherent electrochemical system in relation to the enzymatic kinetics of alpha-amylase was accompanied by the disappearance of the color of the anodic cell as well as a reduction of the electrical signal registered by the instrument.
ISSN:2318-8790