Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences

Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences

Targeted nucleases are powerful genomic tools to precisely change the target genome of living cells, controlling functional genes with high exactness. The clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9) genome editing system has been identified as one of...

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Main Authors: Kamand Tavakoli, Alireza Pour-Aboughadareh, Farzad Kianersi, Peter Poczai, Alireza Etminan, Lia Shooshtari
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:BioTech
Subjects:
CRISPR system
Cas proteins
agriculture
animal science
human disease
Online Access:https://www.mdpi.com/2673-6284/10/3/14
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spelling doaj-4863060dcea443f4a7dc51e8dd5dbdf62021-09-25T23:48:13ZengMDPI AGBioTech2673-62842021-07-0110141410.3390/biotech10030014Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life SciencesKamand Tavakoli0Alireza Pour-Aboughadareh1Farzad Kianersi2Peter Poczai3Alireza Etminan4Lia Shooshtari5Department of Medical Laboratory Science, Kermanshah Branch, Islamic Azad University, Kermanshah P.O. Box 6718997551, IranSeed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj P.O. Box 3158854119, IranDepartment of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan P.O. Box 6517838695, IranBotany Unit, Finnish Museum of Natural History, University of Helsinki, P.O. Box 7, FI-00014 Helsinki, FinlandDepartment of Plant Breeding and Biotechnology, Kermanshah Branch, Islamic Azad University, Kermanshah P.O. Box 6718997551, IranDepartment of Plant Breeding and Biotechnology, Kermanshah Branch, Islamic Azad University, Kermanshah P.O. Box 6718997551, IranTargeted nucleases are powerful genomic tools to precisely change the target genome of living cells, controlling functional genes with high exactness. The clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9) genome editing system has been identified as one of the most useful biological tools in genetic engineering that is taken from adaptive immune strategies for bacteria. In recent years, this system has made significant progress and it has been widely used in genome editing to create gene knock-ins, knock-outs, and point mutations. This paper summarizes the application of this system in various biological sciences, including medicine, plant science, and animal breeding.https://www.mdpi.com/2673-6284/10/3/14CRISPR systemCas proteinsagricultureanimal sciencehuman disease
collection DOAJ
language English
format Article
sources DOAJ
author Kamand Tavakoli
Alireza Pour-Aboughadareh
Farzad Kianersi
Peter Poczai
Alireza Etminan
Lia Shooshtari
spellingShingle Kamand Tavakoli
Alireza Pour-Aboughadareh
Farzad Kianersi
Peter Poczai
Alireza Etminan
Lia Shooshtari
Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences
BioTech
CRISPR system
Cas proteins
agriculture
animal science
human disease
author_facet Kamand Tavakoli
Alireza Pour-Aboughadareh
Farzad Kianersi
Peter Poczai
Alireza Etminan
Lia Shooshtari
author_sort Kamand Tavakoli
title Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences
title_short Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences
title_full Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences
title_fullStr Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences
title_full_unstemmed Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences
title_sort applications of crispr-cas9 as an advanced genome editing system in life sciences
publisher MDPI AG
series BioTech
issn 2673-6284
publishDate 2021-07-01
description Targeted nucleases are powerful genomic tools to precisely change the target genome of living cells, controlling functional genes with high exactness. The clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9) genome editing system has been identified as one of the most useful biological tools in genetic engineering that is taken from adaptive immune strategies for bacteria. In recent years, this system has made significant progress and it has been widely used in genome editing to create gene knock-ins, knock-outs, and point mutations. This paper summarizes the application of this system in various biological sciences, including medicine, plant science, and animal breeding.
topic CRISPR system
Cas proteins
agriculture
animal science
human disease
url https://www.mdpi.com/2673-6284/10/3/14
work_keys_str_mv AT kamandtavakoli applicationsofcrisprcas9asanadvancedgenomeeditingsysteminlifesciences
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AT farzadkianersi applicationsofcrisprcas9asanadvancedgenomeeditingsysteminlifesciences
AT peterpoczai applicationsofcrisprcas9asanadvancedgenomeeditingsysteminlifesciences
AT alirezaetminan applicationsofcrisprcas9asanadvancedgenomeeditingsysteminlifesciences
AT liashooshtari applicationsofcrisprcas9asanadvancedgenomeeditingsysteminlifesciences
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