Utilization of horticultural waste (Apple Pomace) for multiple carbohydrase production from Rhizopus delemar F2 under solid state fermentation

• Main Authors: Shruti Pathania, Nivedita Sharma, Shweta Handa
• Format: Article
• Language: English
• Published: SpringerOpen 2018-06-01
• Series: Journal of Genetic Engineering and Biotechnology
• Online Access: http://www.sciencedirect.com/science/article/pii/S1687157X17300860

The brown rot fungus Rhizopus delemar F2 was shown to produce extracellular thermostable and multiple carbohydrase enzymes. The potential of Rhizopus delemar F2 in utilizing apple pomace under solid state fermentation (SSF) is the purpose of the study. Solid state fermentation (SSF) is a very effective technique opposed to submerged fermentation in various aspects. Enhanced production of multiple carbohydrases 18.20 U g−1 of cellulose, 158.30 U g−1 of xylanase, 61.50 U g−1 of pectinase and amylase 21.03 U g−1 was released by microwave pretreatment of apple pomace at 450 W for 1 min and then by incubation the culture thus obtained at 30 °C for 6 days with moisture content of 1:4.5. Apple pomace can serve as a potential source of raw material for the production of multiple carbohydrases. Besides, it can find great commercial significance in production of bioethanol and various industries like textile, fruit juice, paper and pulp industry. Keywords: Cellulase, Xylanase, Amylase, Pectinase, Acetone precipitation, Waste utilization