Long noncoding RNA DLEU1 aggravates glioma progression via the miR-421/MEF2D axis

• Main Authors: Feng L, He M, Rao M, Diao J, Zhu Y
• Format: Article
• Language: English
• Published: Dove Medical Press 2019-07-01
• Series: OncoTargets and Therapy
• Subjects: Glioma; LncRNA; DLEU1; miR-421; MEF2D
• Online Access: https://www.dovepress.com/long-noncoding-rna-dleu1-aggravates-glioma-progression-via-the-mir-421-peer-reviewed-article-OTT

Li Feng,1,* Mingyuan He,1,* Min Rao,2 Jiandong Diao,3 Yonggang Zhu11Department of Radiotherapy, China-Japan Union Hospital of Jilin University, Changchun 130033, People’s Republic of China; 2Department of Gastroenterology, The First Hospital of Jilin University, Changchun 130021, People’s Republic of China; 3Department of Oncology and Hematology, China-Japan Union Hospital of Jilin University, Changchun 130033, People’s Republic of China*These authors contributed equally to this workBackground: Long noncoding RNA (lncRNA) deleted in lymphocytic leukemia 1 (DLEU1) was reported to be involved in the development and progression of multiple cancers. However, the accurate expression pattern, biological function and potential molecular mechanism of DLEU1 in glioma are not yet known. The present study investigated the role of DLEU in the development and progression of glioma, as well as the potential mechanism played by DLEU1 in glioma.Materials and methods: The levels of DLEUI in glioma tissues and cell lines were examined using quantitative real-time PCR. The potential effects of DLEU1 on the proliferation, mobility, invasion and apoptosis of glioma cells were evaluated using corresponding in vitro experiments. The association between DLEU1 and microRNA (miR)-421 was also determined using luciferase reporter activity and RNA immunoprecipitation (RIP) assays.Results: The results revealed that DLEU1 was significantly upregulated in glioma tissues and cell lines. Increased DLEU1 was positively associated with the high-grade carcinoma (III–IV). Functional studies revealed that knockdown of DLEU1 expression by siRNA led to decreased proliferation, migration and invasion and increased apoptosis in human glioma cells. Furthermore, luciferase reporter activity and RIP assays confirmed that DLEUI could act as a competing endogenous RNA (ceRNA) for miR-421 that functioned as a tumor suppressor in glioma. Moreover, inhibition miR-421 partially restored the effect of DLEU1 knockdown on the glioma cells. DLEU1 could regulate myocyte enhancer factor 2D (MEF2D) expression, a known target of miR-421 in glioma cells.Conclusion: Taken together, these findings suggested that DLEU1 regulated MEF2D expression to promote glioma progression by sponging miR-421 and that DLEU1 might be a potential therapeutic target for glioma.Keywords: glioma, LncRNA, DLEU1, miR-421, MEF2D