Simulation of hypoxia of myocardial cells in microfluidic systems

Abstract The paper presents a newly designed microfluidic system that allows simulation of myocardial hypoxia by biochemical method. The geometry of the microsystem was designed in such a way, that quantitative fluorescent measurements using a spectrofluorometric plate reader was possible. Biochemic...

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Main Authors: Anna Kobuszewska, Elżbieta Jastrzębska, Kamil Żukowski, Zbigniew Brzózka
Format: Article
Language:English
Published: Nature Publishing Group 2020-09-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-020-72660-w
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spelling doaj-4803df6aa5414c97b3eb9a11305ec2ba2021-09-26T11:23:24ZengNature Publishing GroupScientific Reports2045-23222020-09-0110111110.1038/s41598-020-72660-wSimulation of hypoxia of myocardial cells in microfluidic systemsAnna Kobuszewska0Elżbieta Jastrzębska1Kamil Żukowski2Zbigniew Brzózka3Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of TechnologyChair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of TechnologyCEZAMAT, Warsaw University of TechnologyChair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of TechnologyAbstract The paper presents a newly designed microfluidic system that allows simulation of myocardial hypoxia by biochemical method. The geometry of the microsystem was designed in such a way, that quantitative fluorescent measurements using a spectrofluorometric plate reader was possible. Biochemical simulation of hypoxia was carried out using potent mitochondrial oxidative phosphorylation uncoupler—Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP). Two cardiac cell lines were used in the study—rat cardiomyoblasts (H9C2) and human cardiomyocytes. The effectiveness of biochemical simulation of hypoxia was studied using two fluorescent dyes: carbocyanine iodide (JC-1) and Fluo-4. Changes in the mitochondrial membrane potential and concentration of intracellular calcium ions were tested. The major novelty of this research was the applying the microfluidic system to create hypoxia conditions for cardiac cells using the biochemical approach. In further studies, the presented hypoxia model could be used to develop new methods of treatment of ischemic heart disease for example in cell therapy based on stem cells.https://doi.org/10.1038/s41598-020-72660-w
collection DOAJ
language English
format Article
sources DOAJ
author Anna Kobuszewska
Elżbieta Jastrzębska
Kamil Żukowski
Zbigniew Brzózka
spellingShingle Anna Kobuszewska
Elżbieta Jastrzębska
Kamil Żukowski
Zbigniew Brzózka
Simulation of hypoxia of myocardial cells in microfluidic systems
Scientific Reports
author_facet Anna Kobuszewska
Elżbieta Jastrzębska
Kamil Żukowski
Zbigniew Brzózka
author_sort Anna Kobuszewska
title Simulation of hypoxia of myocardial cells in microfluidic systems
title_short Simulation of hypoxia of myocardial cells in microfluidic systems
title_full Simulation of hypoxia of myocardial cells in microfluidic systems
title_fullStr Simulation of hypoxia of myocardial cells in microfluidic systems
title_full_unstemmed Simulation of hypoxia of myocardial cells in microfluidic systems
title_sort simulation of hypoxia of myocardial cells in microfluidic systems
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2020-09-01
description Abstract The paper presents a newly designed microfluidic system that allows simulation of myocardial hypoxia by biochemical method. The geometry of the microsystem was designed in such a way, that quantitative fluorescent measurements using a spectrofluorometric plate reader was possible. Biochemical simulation of hypoxia was carried out using potent mitochondrial oxidative phosphorylation uncoupler—Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP). Two cardiac cell lines were used in the study—rat cardiomyoblasts (H9C2) and human cardiomyocytes. The effectiveness of biochemical simulation of hypoxia was studied using two fluorescent dyes: carbocyanine iodide (JC-1) and Fluo-4. Changes in the mitochondrial membrane potential and concentration of intracellular calcium ions were tested. The major novelty of this research was the applying the microfluidic system to create hypoxia conditions for cardiac cells using the biochemical approach. In further studies, the presented hypoxia model could be used to develop new methods of treatment of ischemic heart disease for example in cell therapy based on stem cells.
url https://doi.org/10.1038/s41598-020-72660-w
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AT elzbietajastrzebska simulationofhypoxiaofmyocardialcellsinmicrofluidicsystems
AT kamilzukowski simulationofhypoxiaofmyocardialcellsinmicrofluidicsystems
AT zbigniewbrzozka simulationofhypoxiaofmyocardialcellsinmicrofluidicsystems
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