Simulation of hypoxia of myocardial cells in microfluidic systems
Abstract The paper presents a newly designed microfluidic system that allows simulation of myocardial hypoxia by biochemical method. The geometry of the microsystem was designed in such a way, that quantitative fluorescent measurements using a spectrofluorometric plate reader was possible. Biochemic...
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2020-09-01
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Online Access: | https://doi.org/10.1038/s41598-020-72660-w |
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doaj-4803df6aa5414c97b3eb9a11305ec2ba2021-09-26T11:23:24ZengNature Publishing GroupScientific Reports2045-23222020-09-0110111110.1038/s41598-020-72660-wSimulation of hypoxia of myocardial cells in microfluidic systemsAnna Kobuszewska0Elżbieta Jastrzębska1Kamil Żukowski2Zbigniew Brzózka3Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of TechnologyChair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of TechnologyCEZAMAT, Warsaw University of TechnologyChair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of TechnologyAbstract The paper presents a newly designed microfluidic system that allows simulation of myocardial hypoxia by biochemical method. The geometry of the microsystem was designed in such a way, that quantitative fluorescent measurements using a spectrofluorometric plate reader was possible. Biochemical simulation of hypoxia was carried out using potent mitochondrial oxidative phosphorylation uncoupler—Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP). Two cardiac cell lines were used in the study—rat cardiomyoblasts (H9C2) and human cardiomyocytes. The effectiveness of biochemical simulation of hypoxia was studied using two fluorescent dyes: carbocyanine iodide (JC-1) and Fluo-4. Changes in the mitochondrial membrane potential and concentration of intracellular calcium ions were tested. The major novelty of this research was the applying the microfluidic system to create hypoxia conditions for cardiac cells using the biochemical approach. In further studies, the presented hypoxia model could be used to develop new methods of treatment of ischemic heart disease for example in cell therapy based on stem cells.https://doi.org/10.1038/s41598-020-72660-w |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anna Kobuszewska Elżbieta Jastrzębska Kamil Żukowski Zbigniew Brzózka |
spellingShingle |
Anna Kobuszewska Elżbieta Jastrzębska Kamil Żukowski Zbigniew Brzózka Simulation of hypoxia of myocardial cells in microfluidic systems Scientific Reports |
author_facet |
Anna Kobuszewska Elżbieta Jastrzębska Kamil Żukowski Zbigniew Brzózka |
author_sort |
Anna Kobuszewska |
title |
Simulation of hypoxia of myocardial cells in microfluidic systems |
title_short |
Simulation of hypoxia of myocardial cells in microfluidic systems |
title_full |
Simulation of hypoxia of myocardial cells in microfluidic systems |
title_fullStr |
Simulation of hypoxia of myocardial cells in microfluidic systems |
title_full_unstemmed |
Simulation of hypoxia of myocardial cells in microfluidic systems |
title_sort |
simulation of hypoxia of myocardial cells in microfluidic systems |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2020-09-01 |
description |
Abstract The paper presents a newly designed microfluidic system that allows simulation of myocardial hypoxia by biochemical method. The geometry of the microsystem was designed in such a way, that quantitative fluorescent measurements using a spectrofluorometric plate reader was possible. Biochemical simulation of hypoxia was carried out using potent mitochondrial oxidative phosphorylation uncoupler—Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP). Two cardiac cell lines were used in the study—rat cardiomyoblasts (H9C2) and human cardiomyocytes. The effectiveness of biochemical simulation of hypoxia was studied using two fluorescent dyes: carbocyanine iodide (JC-1) and Fluo-4. Changes in the mitochondrial membrane potential and concentration of intracellular calcium ions were tested. The major novelty of this research was the applying the microfluidic system to create hypoxia conditions for cardiac cells using the biochemical approach. In further studies, the presented hypoxia model could be used to develop new methods of treatment of ischemic heart disease for example in cell therapy based on stem cells. |
url |
https://doi.org/10.1038/s41598-020-72660-w |
work_keys_str_mv |
AT annakobuszewska simulationofhypoxiaofmyocardialcellsinmicrofluidicsystems AT elzbietajastrzebska simulationofhypoxiaofmyocardialcellsinmicrofluidicsystems AT kamilzukowski simulationofhypoxiaofmyocardialcellsinmicrofluidicsystems AT zbigniewbrzozka simulationofhypoxiaofmyocardialcellsinmicrofluidicsystems |
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