Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma1

Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma1

A method was developed to separate and quantitatively determine two different triglyceride lipase activities in human postheparin plasma: hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL). Affinity chromatography on heparin-Sepharose columns was used for the separation. Rechromatograp...

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Main Authors: J Boberg, J Augustin, M L Baginsky, P Tejada, W V Brown
Format: Article
Language:English
Published: Elsevier 1977-07-01
Series:Journal of Lipid Research
Subjects:
triglyceride lipase activities
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520416736
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spelling doaj-47d671f6e18e4ce6b445419a1f6fc74f2021-04-24T05:53:54ZengElsevierJournal of Lipid Research0022-22751977-07-01184544547Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma1J Boberg0J Augustin1M L Baginsky2P Tejada3W V Brown4Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093; Department of Geriatrics, University of Uppsala, Box 641, S-751 27 Uppsala, SwedenDepartment of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093Department of Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093A method was developed to separate and quantitatively determine two different triglyceride lipase activities in human postheparin plasma: hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL). Affinity chromatography on heparin-Sepharose columns was used for the separation. Rechromatography of purified H-TGL on heparin-Sepharose resulted in recoveries of 74 and 97% of these enzyme activities, respectively. The analytical errors for the determinations of the two activities were 11.4 and 9.6%, respectively.http://www.sciencedirect.com/science/article/pii/S0022227520416736triglyceride lipase activities
collection DOAJ
language English
format Article
sources DOAJ
author J Boberg
J Augustin
M L Baginsky
P Tejada
W V Brown
spellingShingle J Boberg
J Augustin
M L Baginsky
P Tejada
W V Brown
Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma1
Journal of Lipid Research
triglyceride lipase activities
author_facet J Boberg
J Augustin
M L Baginsky
P Tejada
W V Brown
author_sort J Boberg
title Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma1
title_short Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma1
title_full Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma1
title_fullStr Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma1
title_full_unstemmed Quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma1
title_sort quantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma1
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1977-07-01
description A method was developed to separate and quantitatively determine two different triglyceride lipase activities in human postheparin plasma: hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL). Affinity chromatography on heparin-Sepharose columns was used for the separation. Rechromatography of purified H-TGL on heparin-Sepharose resulted in recoveries of 74 and 97% of these enzyme activities, respectively. The analytical errors for the determinations of the two activities were 11.4 and 9.6%, respectively.
topic triglyceride lipase activities
url http://www.sciencedirect.com/science/article/pii/S0022227520416736
work_keys_str_mv AT jboberg quantitativedeterminationofhepaticandlipoproteinlipaseactivitiesfromhumanpostheparinplasma1
AT jaugustin quantitativedeterminationofhepaticandlipoproteinlipaseactivitiesfromhumanpostheparinplasma1
AT mlbaginsky quantitativedeterminationofhepaticandlipoproteinlipaseactivitiesfromhumanpostheparinplasma1
AT ptejada quantitativedeterminationofhepaticandlipoproteinlipaseactivitiesfromhumanpostheparinplasma1
AT wvbrown quantitativedeterminationofhepaticandlipoproteinlipaseactivitiesfromhumanpostheparinplasma1
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