Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR Spectroscopy

Bacterial pathogens expose on the cell surface a variety of complex carbohydrate molecules. Gram-negative bacteria produce lipopolysaccharides, which are the main components of the outer membrane of bacterial envelopes and play a major role in host–pathogen interactions. <i>B. pertussis</i&...

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Main Authors: Karolina Ucieklak, Sabina Koj, Tomasz Niedziela
Format: Article
Language:English
Published: MDPI AG 2021-01-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/22/3/1029
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spelling doaj-47cc2764e244494abf6ed858645643d42021-01-22T00:00:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-01221029102910.3390/ijms22031029Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR SpectroscopyKarolina Ucieklak0Sabina Koj1Tomasz Niedziela2Hirszfeld Institute of Immunology and Experimental Therapy, 53-114 Wroclaw, PolandHirszfeld Institute of Immunology and Experimental Therapy, 53-114 Wroclaw, PolandHirszfeld Institute of Immunology and Experimental Therapy, 53-114 Wroclaw, PolandBacterial pathogens expose on the cell surface a variety of complex carbohydrate molecules. Gram-negative bacteria produce lipopolysaccharides, which are the main components of the outer membrane of bacterial envelopes and play a major role in host–pathogen interactions. <i>B. pertussis</i>, <i>B. parapertussis</i>, <i>B. bronchiseptica,</i> and <i>B. holmesii</i>, are mammalian respiratory pathogens, having substantial economic impact on human health and agriculture. <i>B. pertussis</i> is responsible for whooping cough (pertussis) and <i>B. holmesii</i> is the second pertussis etiological factor, but the current anti-pertussis vaccines do not provide cross-protection. The structural data on any given hypothetical carbohydrate antigen is a prerequisite for further analysis of structure-related activities and their interaction with hosts. <sup>1</sup>H NMR spectra constitute fingerprints of the analyzed glycans and provide unique identity information. The concept of structure-reporter groups has now been augmented by <sup>1</sup>H,<sup>13</sup>C-correlation spectra of the <i>Bordetella</i> oligosaccharides. The comparative analysis of <i>Bordetellae</i> oligosaccharides (OS) revealed that the hexasaccharide, comprising the α-Glc<i>p</i>N, α-Glc<i>p</i>A, 4,6-disubstituted-β-Glc<i>p</i>, 2,7-disubstituted-<span style="font-variant: small-caps;">l</span>-α-<span style="font-variant: small-caps;">d</span>-Hep<i>p</i>, 3,4-disubstituted-<span style="font-variant: small-caps;">l</span>-α-<span style="font-variant: small-caps;">d</span>-Hep<i>p,</i> and Kdo, constitute the least variable OS segment. This minimal common element in the structure of lipopolysaccharides of <i>Bordetellae</i> could be used to devise a universal cross-protective vaccine component against infections with various bacteria from the genus <i>Bordetella</i>.https://www.mdpi.com/1422-0067/22/3/1029<i>Bordetella</i> spp.lipopolysaccharidecore oligosaccharideNMR spectroscopywhooping coughpertussis
collection DOAJ
language English
format Article
sources DOAJ
author Karolina Ucieklak
Sabina Koj
Tomasz Niedziela
spellingShingle Karolina Ucieklak
Sabina Koj
Tomasz Niedziela
Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR Spectroscopy
International Journal of Molecular Sciences
<i>Bordetella</i> spp.
lipopolysaccharide
core oligosaccharide
NMR spectroscopy
whooping cough
pertussis
author_facet Karolina Ucieklak
Sabina Koj
Tomasz Niedziela
author_sort Karolina Ucieklak
title Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR Spectroscopy
title_short Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR Spectroscopy
title_full Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR Spectroscopy
title_fullStr Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR Spectroscopy
title_full_unstemmed Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR Spectroscopy
title_sort conserved structural features of core oligosaccharides among the lipopolysaccharides of respiratory pathogens from the genus <i>bordetella</i> analyzed exclusively by nmr spectroscopy
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1661-6596
1422-0067
publishDate 2021-01-01
description Bacterial pathogens expose on the cell surface a variety of complex carbohydrate molecules. Gram-negative bacteria produce lipopolysaccharides, which are the main components of the outer membrane of bacterial envelopes and play a major role in host–pathogen interactions. <i>B. pertussis</i>, <i>B. parapertussis</i>, <i>B. bronchiseptica,</i> and <i>B. holmesii</i>, are mammalian respiratory pathogens, having substantial economic impact on human health and agriculture. <i>B. pertussis</i> is responsible for whooping cough (pertussis) and <i>B. holmesii</i> is the second pertussis etiological factor, but the current anti-pertussis vaccines do not provide cross-protection. The structural data on any given hypothetical carbohydrate antigen is a prerequisite for further analysis of structure-related activities and their interaction with hosts. <sup>1</sup>H NMR spectra constitute fingerprints of the analyzed glycans and provide unique identity information. The concept of structure-reporter groups has now been augmented by <sup>1</sup>H,<sup>13</sup>C-correlation spectra of the <i>Bordetella</i> oligosaccharides. The comparative analysis of <i>Bordetellae</i> oligosaccharides (OS) revealed that the hexasaccharide, comprising the α-Glc<i>p</i>N, α-Glc<i>p</i>A, 4,6-disubstituted-β-Glc<i>p</i>, 2,7-disubstituted-<span style="font-variant: small-caps;">l</span>-α-<span style="font-variant: small-caps;">d</span>-Hep<i>p</i>, 3,4-disubstituted-<span style="font-variant: small-caps;">l</span>-α-<span style="font-variant: small-caps;">d</span>-Hep<i>p,</i> and Kdo, constitute the least variable OS segment. This minimal common element in the structure of lipopolysaccharides of <i>Bordetellae</i> could be used to devise a universal cross-protective vaccine component against infections with various bacteria from the genus <i>Bordetella</i>.
topic <i>Bordetella</i> spp.
lipopolysaccharide
core oligosaccharide
NMR spectroscopy
whooping cough
pertussis
url https://www.mdpi.com/1422-0067/22/3/1029
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