Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR Spectroscopy
Bacterial pathogens expose on the cell surface a variety of complex carbohydrate molecules. Gram-negative bacteria produce lipopolysaccharides, which are the main components of the outer membrane of bacterial envelopes and play a major role in host–pathogen interactions. <i>B. pertussis</i&...
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doaj-47cc2764e244494abf6ed858645643d42021-01-22T00:00:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-01221029102910.3390/ijms22031029Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR SpectroscopyKarolina Ucieklak0Sabina Koj1Tomasz Niedziela2Hirszfeld Institute of Immunology and Experimental Therapy, 53-114 Wroclaw, PolandHirszfeld Institute of Immunology and Experimental Therapy, 53-114 Wroclaw, PolandHirszfeld Institute of Immunology and Experimental Therapy, 53-114 Wroclaw, PolandBacterial pathogens expose on the cell surface a variety of complex carbohydrate molecules. Gram-negative bacteria produce lipopolysaccharides, which are the main components of the outer membrane of bacterial envelopes and play a major role in host–pathogen interactions. <i>B. pertussis</i>, <i>B. parapertussis</i>, <i>B. bronchiseptica,</i> and <i>B. holmesii</i>, are mammalian respiratory pathogens, having substantial economic impact on human health and agriculture. <i>B. pertussis</i> is responsible for whooping cough (pertussis) and <i>B. holmesii</i> is the second pertussis etiological factor, but the current anti-pertussis vaccines do not provide cross-protection. The structural data on any given hypothetical carbohydrate antigen is a prerequisite for further analysis of structure-related activities and their interaction with hosts. <sup>1</sup>H NMR spectra constitute fingerprints of the analyzed glycans and provide unique identity information. The concept of structure-reporter groups has now been augmented by <sup>1</sup>H,<sup>13</sup>C-correlation spectra of the <i>Bordetella</i> oligosaccharides. The comparative analysis of <i>Bordetellae</i> oligosaccharides (OS) revealed that the hexasaccharide, comprising the α-Glc<i>p</i>N, α-Glc<i>p</i>A, 4,6-disubstituted-β-Glc<i>p</i>, 2,7-disubstituted-<span style="font-variant: small-caps;">l</span>-α-<span style="font-variant: small-caps;">d</span>-Hep<i>p</i>, 3,4-disubstituted-<span style="font-variant: small-caps;">l</span>-α-<span style="font-variant: small-caps;">d</span>-Hep<i>p,</i> and Kdo, constitute the least variable OS segment. This minimal common element in the structure of lipopolysaccharides of <i>Bordetellae</i> could be used to devise a universal cross-protective vaccine component against infections with various bacteria from the genus <i>Bordetella</i>.https://www.mdpi.com/1422-0067/22/3/1029<i>Bordetella</i> spp.lipopolysaccharidecore oligosaccharideNMR spectroscopywhooping coughpertussis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Karolina Ucieklak Sabina Koj Tomasz Niedziela |
spellingShingle |
Karolina Ucieklak Sabina Koj Tomasz Niedziela Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR Spectroscopy International Journal of Molecular Sciences <i>Bordetella</i> spp. lipopolysaccharide core oligosaccharide NMR spectroscopy whooping cough pertussis |
author_facet |
Karolina Ucieklak Sabina Koj Tomasz Niedziela |
author_sort |
Karolina Ucieklak |
title |
Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR Spectroscopy |
title_short |
Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR Spectroscopy |
title_full |
Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR Spectroscopy |
title_fullStr |
Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR Spectroscopy |
title_full_unstemmed |
Conserved Structural Features of Core Oligosaccharides among the Lipopolysaccharides of Respiratory Pathogens from the Genus <i>Bordetella</i> Analyzed Exclusively by NMR Spectroscopy |
title_sort |
conserved structural features of core oligosaccharides among the lipopolysaccharides of respiratory pathogens from the genus <i>bordetella</i> analyzed exclusively by nmr spectroscopy |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-01-01 |
description |
Bacterial pathogens expose on the cell surface a variety of complex carbohydrate molecules. Gram-negative bacteria produce lipopolysaccharides, which are the main components of the outer membrane of bacterial envelopes and play a major role in host–pathogen interactions. <i>B. pertussis</i>, <i>B. parapertussis</i>, <i>B. bronchiseptica,</i> and <i>B. holmesii</i>, are mammalian respiratory pathogens, having substantial economic impact on human health and agriculture. <i>B. pertussis</i> is responsible for whooping cough (pertussis) and <i>B. holmesii</i> is the second pertussis etiological factor, but the current anti-pertussis vaccines do not provide cross-protection. The structural data on any given hypothetical carbohydrate antigen is a prerequisite for further analysis of structure-related activities and their interaction with hosts. <sup>1</sup>H NMR spectra constitute fingerprints of the analyzed glycans and provide unique identity information. The concept of structure-reporter groups has now been augmented by <sup>1</sup>H,<sup>13</sup>C-correlation spectra of the <i>Bordetella</i> oligosaccharides. The comparative analysis of <i>Bordetellae</i> oligosaccharides (OS) revealed that the hexasaccharide, comprising the α-Glc<i>p</i>N, α-Glc<i>p</i>A, 4,6-disubstituted-β-Glc<i>p</i>, 2,7-disubstituted-<span style="font-variant: small-caps;">l</span>-α-<span style="font-variant: small-caps;">d</span>-Hep<i>p</i>, 3,4-disubstituted-<span style="font-variant: small-caps;">l</span>-α-<span style="font-variant: small-caps;">d</span>-Hep<i>p,</i> and Kdo, constitute the least variable OS segment. This minimal common element in the structure of lipopolysaccharides of <i>Bordetellae</i> could be used to devise a universal cross-protective vaccine component against infections with various bacteria from the genus <i>Bordetella</i>. |
topic |
<i>Bordetella</i> spp. lipopolysaccharide core oligosaccharide NMR spectroscopy whooping cough pertussis |
url |
https://www.mdpi.com/1422-0067/22/3/1029 |
work_keys_str_mv |
AT karolinaucieklak conservedstructuralfeaturesofcoreoligosaccharidesamongthelipopolysaccharidesofrespiratorypathogensfromthegenusibordetellaianalyzedexclusivelybynmrspectroscopy AT sabinakoj conservedstructuralfeaturesofcoreoligosaccharidesamongthelipopolysaccharidesofrespiratorypathogensfromthegenusibordetellaianalyzedexclusivelybynmrspectroscopy AT tomaszniedziela conservedstructuralfeaturesofcoreoligosaccharidesamongthelipopolysaccharidesofrespiratorypathogensfromthegenusibordetellaianalyzedexclusivelybynmrspectroscopy |
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