Novel Template Plasmids pCyaA’-Kan and pCyaA’-Cam for Generation of Unmarked Chromosomal <i>cyaA</i>’ Translational Fusion to T3SS Effectors in <i>Salmonella</i>

Novel Template Plasmids pCyaA’-Kan and pCyaA’-Cam for Generation of Unmarked Chromosomal <i>cyaA</i>’ Translational Fusion to T3SS Effectors in <i>Salmonella</i>

The type III secretion systems (T3SS) encoded in pathogenicity islands SPI-1 and SPI-2 are key virulence factors of <i>Salmonella</i>. These systems translocate proteins known as effectors into eukaryotic cells during infection. To characterize the functionality of T3SS effectors, gene f...

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Bibliographic Details
Main Authors: Paulina A. Fernández, Marcela Zabner, Jaime Ortega, Constanza Morgado, Fernando Amaya, Gabriel Vera, Carolina Rubilar, Beatriz Salas, Víctor Cuevas, Camila Valenzuela, Fernando Baisón-Olmo, Sergio A. Álvarez, Carlos A. Santiviago
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:Microorganisms
Subjects:
<i>Salmonella</i>
T3SS
SPI-1
SPI-2
effector
translational fusion
Online Access:https://www.mdpi.com/2076-2607/9/3/475

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