Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats
Nephrotoxicity is a major limiting factor in cisplatin treatment. In the present study hydro-ethanolic leaf extract of Emblica officinalis was investigated for its protective role in cisplatin induced nephrotoxicity. The experiment was designed for 14 days and male Wistar rats were divided into 9 gr...
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doaj-47bc54338de24a43a8386ff5c2afee122020-11-25T01:53:23ZengElsevierToxicology Reports2214-75002018-01-015270277Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in RatsRupal Purena0Rohit Seth1Renu Bhatt2Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, Bilaspur, 495009, Chhattisgarh, IndiaDepartment of Zoology, Guru Ghasidas Vishwavidyalaya, Bilaspur, 495009, Chhattisgarh, IndiaDepartment of Biotechnology, Guru Ghasidas Vishwavidyalaya, Bilaspur, 495009, Chhattisgarh, India; Corresponding author at: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, Koni, Bilaspur, 495009, Chhattisgarh, India.Nephrotoxicity is a major limiting factor in cisplatin treatment. In the present study hydro-ethanolic leaf extract of Emblica officinalis was investigated for its protective role in cisplatin induced nephrotoxicity. The experiment was designed for 14 days and male Wistar rats were divided into 9 groups (n = 5). Group 1 served as control (with no treatment), group 2 served as a vehicle control and received 0.9% NaCl intraperitoneally (i.p.) on 11th day of the treatment, group 3 received a single dose of cisplatin on 11th day (12 mg/kg body weight, i.p.), group 4–6 received leaf extract only (100 mg/kg, 200 mg/kg and 400 mg/kg body weight, respectively) throughout the treatment, group 7–9 received leaf extract (100 mg/kg, 200 mg/kg and 400 mg/kg body weight, respectively) throughout the treatment and single dose of cisplatin on the 11th day of the leaf extract treatment. At the end of the experiment (i.e. on 14th day) blood samples were collected from all the groups and were sacrificed to study renal functional parameters. Treatment with above doses of E. officinalis leaf extract significantly (p ≤ 0.05) attenuates renal damage by decreasing serum creatinine and blood urea nitrogen (BUN), enhanced the activities of Catalase, SOD, GPx, GR and decreased the renal MDA level compared with the cisplatin treatment group. Furthermore the oral administration of Amla leaf extract improves histological damage and morphological changes in RBCs. Our results suggest that, leaf extract of E. officinalis may ameliorate renal damage caused by cisplatin. Keywords: Emblica officinalis, Cisplatin, Nephrotoxicity, Oxidative stress, Ratshttp://www.sciencedirect.com/science/article/pii/S2214750018300507 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rupal Purena Rohit Seth Renu Bhatt |
spellingShingle |
Rupal Purena Rohit Seth Renu Bhatt Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats Toxicology Reports |
author_facet |
Rupal Purena Rohit Seth Renu Bhatt |
author_sort |
Rupal Purena |
title |
Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats |
title_short |
Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats |
title_full |
Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats |
title_fullStr |
Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats |
title_full_unstemmed |
Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats |
title_sort |
protective role of emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in rats |
publisher |
Elsevier |
series |
Toxicology Reports |
issn |
2214-7500 |
publishDate |
2018-01-01 |
description |
Nephrotoxicity is a major limiting factor in cisplatin treatment. In the present study hydro-ethanolic leaf extract of Emblica officinalis was investigated for its protective role in cisplatin induced nephrotoxicity. The experiment was designed for 14 days and male Wistar rats were divided into 9 groups (n = 5). Group 1 served as control (with no treatment), group 2 served as a vehicle control and received 0.9% NaCl intraperitoneally (i.p.) on 11th day of the treatment, group 3 received a single dose of cisplatin on 11th day (12 mg/kg body weight, i.p.), group 4–6 received leaf extract only (100 mg/kg, 200 mg/kg and 400 mg/kg body weight, respectively) throughout the treatment, group 7–9 received leaf extract (100 mg/kg, 200 mg/kg and 400 mg/kg body weight, respectively) throughout the treatment and single dose of cisplatin on the 11th day of the leaf extract treatment. At the end of the experiment (i.e. on 14th day) blood samples were collected from all the groups and were sacrificed to study renal functional parameters. Treatment with above doses of E. officinalis leaf extract significantly (p ≤ 0.05) attenuates renal damage by decreasing serum creatinine and blood urea nitrogen (BUN), enhanced the activities of Catalase, SOD, GPx, GR and decreased the renal MDA level compared with the cisplatin treatment group. Furthermore the oral administration of Amla leaf extract improves histological damage and morphological changes in RBCs. Our results suggest that, leaf extract of E. officinalis may ameliorate renal damage caused by cisplatin. Keywords: Emblica officinalis, Cisplatin, Nephrotoxicity, Oxidative stress, Rats |
url |
http://www.sciencedirect.com/science/article/pii/S2214750018300507 |
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