Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats

Nephrotoxicity is a major limiting factor in cisplatin treatment. In the present study hydro-ethanolic leaf extract of Emblica officinalis was investigated for its protective role in cisplatin induced nephrotoxicity. The experiment was designed for 14 days and male Wistar rats were divided into 9 gr...

Full description

Bibliographic Details
Main Authors: Rupal Purena, Rohit Seth, Renu Bhatt
Format: Article
Language:English
Published: Elsevier 2018-01-01
Series:Toxicology Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2214750018300507
id doaj-47bc54338de24a43a8386ff5c2afee12
record_format Article
spelling doaj-47bc54338de24a43a8386ff5c2afee122020-11-25T01:53:23ZengElsevierToxicology Reports2214-75002018-01-015270277Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in RatsRupal Purena0Rohit Seth1Renu Bhatt2Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, Bilaspur, 495009, Chhattisgarh, IndiaDepartment of Zoology, Guru Ghasidas Vishwavidyalaya, Bilaspur, 495009, Chhattisgarh, IndiaDepartment of Biotechnology, Guru Ghasidas Vishwavidyalaya, Bilaspur, 495009, Chhattisgarh, India; Corresponding author at: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, Koni, Bilaspur, 495009, Chhattisgarh, India.Nephrotoxicity is a major limiting factor in cisplatin treatment. In the present study hydro-ethanolic leaf extract of Emblica officinalis was investigated for its protective role in cisplatin induced nephrotoxicity. The experiment was designed for 14 days and male Wistar rats were divided into 9 groups (n = 5). Group 1 served as control (with no treatment), group 2 served as a vehicle control and received 0.9% NaCl intraperitoneally (i.p.) on 11th day of the treatment, group 3 received a single dose of cisplatin on 11th day (12 mg/kg body weight, i.p.), group 4–6 received leaf extract only (100 mg/kg, 200 mg/kg and 400 mg/kg body weight, respectively) throughout the treatment, group 7–9 received leaf extract (100 mg/kg, 200 mg/kg and 400 mg/kg body weight, respectively) throughout the treatment and single dose of cisplatin on the 11th day of the leaf extract treatment. At the end of the experiment (i.e. on 14th day) blood samples were collected from all the groups and were sacrificed to study renal functional parameters. Treatment with above doses of E. officinalis leaf extract significantly (p ≤ 0.05) attenuates renal damage by decreasing serum creatinine and blood urea nitrogen (BUN), enhanced the activities of Catalase, SOD, GPx, GR and decreased the renal MDA level compared with the cisplatin treatment group. Furthermore the oral administration of Amla leaf extract improves histological damage and morphological changes in RBCs. Our results suggest that, leaf extract of E. officinalis may ameliorate renal damage caused by cisplatin. Keywords: Emblica officinalis, Cisplatin, Nephrotoxicity, Oxidative stress, Ratshttp://www.sciencedirect.com/science/article/pii/S2214750018300507
collection DOAJ
language English
format Article
sources DOAJ
author Rupal Purena
Rohit Seth
Renu Bhatt
spellingShingle Rupal Purena
Rohit Seth
Renu Bhatt
Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats
Toxicology Reports
author_facet Rupal Purena
Rohit Seth
Renu Bhatt
author_sort Rupal Purena
title Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats
title_short Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats
title_full Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats
title_fullStr Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats
title_full_unstemmed Protective role of Emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in Rats
title_sort protective role of emblica officinalis hydro-ethanolic leaf extract in cisplatin induced nephrotoxicity in rats
publisher Elsevier
series Toxicology Reports
issn 2214-7500
publishDate 2018-01-01
description Nephrotoxicity is a major limiting factor in cisplatin treatment. In the present study hydro-ethanolic leaf extract of Emblica officinalis was investigated for its protective role in cisplatin induced nephrotoxicity. The experiment was designed for 14 days and male Wistar rats were divided into 9 groups (n = 5). Group 1 served as control (with no treatment), group 2 served as a vehicle control and received 0.9% NaCl intraperitoneally (i.p.) on 11th day of the treatment, group 3 received a single dose of cisplatin on 11th day (12 mg/kg body weight, i.p.), group 4–6 received leaf extract only (100 mg/kg, 200 mg/kg and 400 mg/kg body weight, respectively) throughout the treatment, group 7–9 received leaf extract (100 mg/kg, 200 mg/kg and 400 mg/kg body weight, respectively) throughout the treatment and single dose of cisplatin on the 11th day of the leaf extract treatment. At the end of the experiment (i.e. on 14th day) blood samples were collected from all the groups and were sacrificed to study renal functional parameters. Treatment with above doses of E. officinalis leaf extract significantly (p ≤ 0.05) attenuates renal damage by decreasing serum creatinine and blood urea nitrogen (BUN), enhanced the activities of Catalase, SOD, GPx, GR and decreased the renal MDA level compared with the cisplatin treatment group. Furthermore the oral administration of Amla leaf extract improves histological damage and morphological changes in RBCs. Our results suggest that, leaf extract of E. officinalis may ameliorate renal damage caused by cisplatin. Keywords: Emblica officinalis, Cisplatin, Nephrotoxicity, Oxidative stress, Rats
url http://www.sciencedirect.com/science/article/pii/S2214750018300507
work_keys_str_mv AT rupalpurena protectiveroleofemblicaofficinalishydroethanolicleafextractincisplatininducednephrotoxicityinrats
AT rohitseth protectiveroleofemblicaofficinalishydroethanolicleafextractincisplatininducednephrotoxicityinrats
AT renubhatt protectiveroleofemblicaofficinalishydroethanolicleafextractincisplatininducednephrotoxicityinrats
_version_ 1724991161304612864