CALIBRATION OF DENSITOMETRY IN RADIO-ISOTOPIC IN SITU HYBRIDIZATION

CALIBRATION OF DENSITOMETRY IN RADIO-ISOTOPIC IN SITU HYBRIDIZATION

Densitometry on autoradiographs of sections processed for in situ hybridization provides a direct measure for the in situ quantification of mRNA. Gelatin spots, containing different concentrations of the radioisotope, and processed in parallel with the tissue sections, can be used as a sensitive mod...

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Bibliographic Details
Main Authors: Jan M Ruijter, Jaco Hagoort, Piet AJ De Boer, Antoon FM Moorman
Format: Article
Language:English
Published: Slovenian Society for Stereology and Quantitative Image Analysis 2011-05-01
Series:Image Analysis and Stereology
Subjects:
calibration
densitometry
quantitative autoradiography
Online Access:http://www.ias-iss.org/ojs/IAS/article/view/683

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