Effect of kinetin and chloramphenicol on chlorophyll synthesis and chloroplast development in detached lupin cotyledons under low light intensity

• Main Authors: Fortunat Młodzianowski, Ewa Gezela
• Format: Article
• Language: English
• Published: Polish Botanical Society 2015-01-01
• Series: Acta Societatis Botanicorum Poloniae
• Online Access: https://pbsociety.org.pl/journals/index.php/asbp/article/view/5072

Chlorophyll synthesis in detached lupin cotyledons under low light intensity was stimulated by kinetin at 20 mg/l and inhibited by chloramphenicol at 50 mg/1. Kinetin not only conteracted the inhibitory effect of chloramphenicol, but stimulated1 the chlorophyll syntesis to a greater level than in the control material. Kinetin accelerated the starch degradation and the development of chloroplast; its prolonged, action, however, produced some abnormalities, such as an excessive growth of plastids resulting in some cases in bursting of their envelopes, the formation and release f r om plastids of numerous membrane - bound bodies and the accumulation in released and swollen thylakoids of electron - dense substance. In the presence of chloramphenicol, some disturbances in structure of the stroma thylakoids and the appearance of vesicular structures in the stroma and the enlargement of grana and swelling of their thylakoids were observed. Kinetin prevented some of these abnormalities.