Diamine oxidase-modified screen-printed electrode for the redox-mediated determination of histamine

• Main Authors: Ricarda Torre, Estefanía Costa-Rama, Henri P. A. Nouws, Cristina Delerue-Matos
• Format: Article
• Language: English
• Published: SpringerOpen 2020-02-01
• Series: Journal of Analytical Science and Technology
• Subjects: Amperometric biosensor; Enzymatic sensor; Biogenic amine; Histamine; Diamine oxidase; Redox mediator
• Online Access: https://doi.org/10.1186/s40543-020-0203-3

Abstract Histamine is an important biogenic amine because of its role in immune responses and the regulation of physiological functions. It is also used as a food freshness indicator, so its maximum concentration in fish is legally regulated. Although several robust and sensitive methods for histamine detection are already available, it continues to be a challenge to develop simple and portable devices that allow rapid histamine screening at any point of the fish production chain. Thus, in this work, a simple, miniaturized and low-cost sensor for histamine analysis was developed. The construction of the sensor only takes 30 min and consists of the immobilization of the enzyme diamine oxidase on the surface of a screen-printed carbon electrode by cross-linking. The quantification of histamine was achieved by chronoamperometry (+ 0.2V, 120 s) using hexacyanoferrate (III) as a redox mediator. This selective sensor provided a low limit of detection (0.97 mg L−1) and accurate and precise results and was successfully applied to the analysis of spiked tuna and mackerel extracts, obtaining recovery values of 99–100%. Moreover, the sensor shows good stability, maintaining 87.7% of its initial signal after 35 days.