Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines

Cancer therapy has evolved to a more targeted approach and often involves drug combinations to achieve better response rates. Non-thermal plasma (NTP), a technology rapidly expanding its application in the medical field, is a near room temperature ionized gas capable of producing reactive species, a...

Full description

Bibliographic Details
Main Authors:	Julie Lafontaine, Jean-Sébastien Boisvert, Audrey Glory, Sylvain Coulombe, Philip Wong
Format:	Article
Language:	English
Published:	MDPI AG 2020-02-01
Series:	Cancers
Subjects:	radiation therapy non-thermal plasma radio-frequency discharge breast cancer parp-inhibitor olaparib dna-damage
Online Access:	https://www.mdpi.com/2072-6694/12/2/348

id	doaj-4770e6f1dd3c4fefa9de6fa265c0c2dc
record_format	Article
spelling	doaj-4770e6f1dd3c4fefa9de6fa265c0c2dc2020-11-25T02:03:23ZengMDPI AGCancers2072-66942020-02-0112234810.3390/cancers12020348cancers12020348Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell LinesJulie Lafontaine0Jean-Sébastien Boisvert1Audrey Glory2Sylvain Coulombe3Philip Wong4Institut du Cancer de Montréal, CRCHUM, 900 Rue St. Denis, Montreal, QC H2X 0A9, CanadaInstitut du Cancer de Montréal, CRCHUM, 900 Rue St. Denis, Montreal, QC H2X 0A9, CanadaInstitut du Cancer de Montréal, CRCHUM, 900 Rue St. Denis, Montreal, QC H2X 0A9, CanadaPlasma Processing Laboratory, Department of Chemical Engineering, McGill University, 3610 University Street, Montreal, QC H3A 0C5, CanadaInstitut du Cancer de Montréal, CRCHUM, 900 Rue St. Denis, Montreal, QC H2X 0A9, CanadaCancer therapy has evolved to a more targeted approach and often involves drug combinations to achieve better response rates. Non-thermal plasma (NTP), a technology rapidly expanding its application in the medical field, is a near room temperature ionized gas capable of producing reactive species, and can induce cancer cell death both in vitro and <i>in vivo</i>. Here, we used proliferation assay to characterize the plasma sensitivity of fourteen breast cancer cell lines. These assays showed that all tested cell lines were sensitive to NTP. In addition, a good correlation was found comparing cell sensitivity to NTP and radiation therapy (RT), where cells that were sensitive to RT were also sensitive to plasma. Moreover, in some breast cancer cell lines, NTP and RT have a synergistic effect. Adding a dose of PARP-inhibitor olaparib to NTP treatment always increases the efficacy of the treatment. Olaparib also exhibits a synergistic effect with NTP, especially in triple negative breast cancer cells. Results presented here help elucidate the position of plasma use as a potential breast cancer treatment.https://www.mdpi.com/2072-6694/12/2/348radiation therapynon-thermal plasmaradio-frequency dischargebreast cancerparp-inhibitorolaparibdna-damage
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Julie Lafontaine Jean-Sébastien Boisvert Audrey Glory Sylvain Coulombe Philip Wong
spellingShingle	Julie Lafontaine Jean-Sébastien Boisvert Audrey Glory Sylvain Coulombe Philip Wong Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines Cancers radiation therapy non-thermal plasma radio-frequency discharge breast cancer parp-inhibitor olaparib dna-damage
author_facet	Julie Lafontaine Jean-Sébastien Boisvert Audrey Glory Sylvain Coulombe Philip Wong
author_sort	Julie Lafontaine
title	Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines
title_short	Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines
title_full	Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines
title_fullStr	Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines
title_full_unstemmed	Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines
title_sort	synergy between non-thermal plasma with radiation therapy and olaparib in a panel of breast cancer cell lines
publisher	MDPI AG
series	Cancers
issn	2072-6694
publishDate	2020-02-01
description	Cancer therapy has evolved to a more targeted approach and often involves drug combinations to achieve better response rates. Non-thermal plasma (NTP), a technology rapidly expanding its application in the medical field, is a near room temperature ionized gas capable of producing reactive species, and can induce cancer cell death both in vitro and <i>in vivo</i>. Here, we used proliferation assay to characterize the plasma sensitivity of fourteen breast cancer cell lines. These assays showed that all tested cell lines were sensitive to NTP. In addition, a good correlation was found comparing cell sensitivity to NTP and radiation therapy (RT), where cells that were sensitive to RT were also sensitive to plasma. Moreover, in some breast cancer cell lines, NTP and RT have a synergistic effect. Adding a dose of PARP-inhibitor olaparib to NTP treatment always increases the efficacy of the treatment. Olaparib also exhibits a synergistic effect with NTP, especially in triple negative breast cancer cells. Results presented here help elucidate the position of plasma use as a potential breast cancer treatment.
topic	radiation therapy non-thermal plasma radio-frequency discharge breast cancer parp-inhibitor olaparib dna-damage
url	https://www.mdpi.com/2072-6694/12/2/348
work_keys_str_mv	AT julielafontaine synergybetweennonthermalplasmawithradiationtherapyandolaparibinapanelofbreastcancercelllines AT jeansebastienboisvert synergybetweennonthermalplasmawithradiationtherapyandolaparibinapanelofbreastcancercelllines AT audreyglory synergybetweennonthermalplasmawithradiationtherapyandolaparibinapanelofbreastcancercelllines AT sylvaincoulombe synergybetweennonthermalplasmawithradiationtherapyandolaparibinapanelofbreastcancercelllines AT philipwong synergybetweennonthermalplasmawithradiationtherapyandolaparibinapanelofbreastcancercelllines
_version_	1724948603561050112

Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines

Similar Items