Determination of No-Observed-Adverse-Effect Level Ammonia in White Mice Through CD4 Expression

• Main Authors: Abdul Rohim Tualeka, Jihan Faradisha, Rizky Maharja
• Format: Article
• Language: English
• Published: SAGE Publishing 2018-10-01
• Series: Dose-Response
• Online Access: https://doi.org/10.1177/1559325818807790

Background: Ammonia is one of the chemical compounds that can cause acute and chronic effects to the workers. The purpose of this study is to determine the no-observed-adverse-effect level (NOAEL) ammonia in white mice. Methods: This study applied a laboratory experimental with design post test-only control group design using white mice as an object. The process of finding the safe limit began by determining the highest dose and the lowest dose of ammonia without effect in white mice. Results: The latest research showed that the highest dose without effect is 0.0456 mg/kg and the lowest dose without effect is 0.0046 mg/kg. After determining the highest and the lowest dose of ammonia, the researcher started to determine the middle dose. The middle dose is the dose between the highest and the lowest doses with the multiplication of 1.5 starting from the lowest dose (there are 5 middle-dose variations from 5 middle concentration variations), consisting of 0.0872, 0.1309, 0.1963, 0.2944, and 0.4416 mg/m 3 . Conclusion: From the result of the middle dose and the lowest dose without effect, it is found the value of the highest NH 3 dose without effect, which showed the highest Index Remmele Scale (IRS) CD4 before the reduction of IRS. The study concludes that the NOAEL of ammonia contained in white mice is 0.0103 mg/kg body weight of the mice.