Insulin-Like Growth Factor Axis Expression in Dental Pulp Cells Derived From Carious Teeth

• Main Authors: Hanaa Esa Alkharobi, Hasanain Al-Khafaji, James Beattie, Deirdre Ann Devine, Reem El-Gendy
• Format: Article
• Language: English
• Published: Frontiers Media S.A. 2018-04-01
• Series: Frontiers in Bioengineering and Biotechnology
• Subjects: insulin-like growth factor axis; IGF binding protein-3; IGF binding proteins-2; dental pulp stem cells; inflammation; pulp regeneration
• Online Access: http://journal.frontiersin.org/article/10.3389/fbioe.2018.00036/full

The insulin-like growth factor (IGF) axis plays an important role in dental tissue regeneration and most components of this axis are expressed in human dental pulp cells (DPCs). In our previous study, we analyzed IGF axis gene expression in DPCs and demonstrated a novel role of IGF binding protein (IGFBP)-2 and -3 in coordinating mineralized matrix formation in differentiating DPCs. A more recent study from our laboratory partially characterized dental pulp stem cells from teeth with superficial caries (cDPCs) and showed that their potential to differentiate odontoblasts and/or into osteoblasts is enhanced by exposure to the mild inflammatory conditions characteristic of superficial caries. In the present study, we examine whether changes apparent in IGF axis expression during osteogenic differentiation of healthy DPCs are also apparent in DPCs derived from carious affected teeth.