Isomer-Specific Effects of <i>cis</i>-9,<i>trans</i>-11- and <i>trans</i>-10,<i>cis</i>-12-CLA on Immune Regulation in Ruminal Epithelial Cells

• Main Authors: Chunlei Yang, Binna Zhu, Shijie Ye, Zhengwei Fu, Jinjun Li
• Format: Article
• Language: English
• Published: MDPI AG 2021-04-01
• Series: Animals
• Subjects: <i>cis</i>-9,<i>trans</i>-11-CLA; <i>trans</i>-10,<i>cis</i>-12-CLA; immunoregulation; lipid metabolism; ruminal epithelial cells
• Online Access: https://www.mdpi.com/2076-2615/11/4/1169

In this study, we used transcriptomics and qPCR to investigate the potential immunoprotective effects of different conjugated linoleic acid (CLA) isomers, the natural rumen microbial metabolites, on lipopolysaccharide (LPS)-induced inflammation of ruminal epithelial cells (RECs) in vitro. The results showed that 100 μM <i>trans</i>-10,<i>cis</i>-12-CLA exerted higher anti-inflammatory effects than <i>cis</i>-9,<i>trans</i>-11-CLA by significantly downregulating the expression of genes related to inflammation, cell proliferation and migration in RECs upon LPS stimulation. Transcriptomic analyses further indicated that pretreatment with <i>trans</i>-10,<i>cis</i>-12-CLA, but not <i>cis</i>-9,<i>trans</i>-11-CLA, significantly suppressed the biological signals of GO terms’ response to LPS, the regulation of signal transduction and cytokine production and KEGG pathways NF-κB, chemokine, NOD-like receptor, Hippo, PI3K-Akt, TGF-β and Rap1 signaling in RECs upon LPS stimulation. Furthermore, pretreatment with <i>trans</i>-10,<i>cis</i>-12-CLA significantly reduced the expression of lipogenic genes and the biosynthesis of the unsaturated fatty acid pathway in RECs compared with the LPS group, however, <i>cis</i>-9,<i>trans</i>-11-CLA exhibited the opposite results. These results suggest the distinct isomer differences of CLA in the regulation of inflammatory responses and adipocytokine signaling in RECs and will provide important references for determining their target use in the future.