INTERRELATION OF NITROGEN NUTRITION AND GLYCOGEN ACCUMULATION IN THE CELLS OF CHLOROBIUM LIMICOLA

• Main Authors: О. В. Левицька, М. Б. Горішний, С. П. Гудзь
• Format: Article
• Language: English
• Published: Odessa I. I. Mechnikov National University 2010-03-01
• Series: Mìkrobìologìâ ì Bìotehnologìâ
• Subjects: зелені сіркобактерії; chlorobium limicola; азотфіксація; глікоген; джерела азоту
• Online Access: http://mbt.onu.edu.ua/article/view/98515

Ability of green sulfur bacteria Chlorobium limicola Ya-2002 to assimilate various nitrogen sources was investigated. It was shown that Chlorobium utilize nitrogen of ammonium, amino group and fixes molecular nitrogen. Nitrogen of ammonium was found to be the most suitable for these bacteria. Addition of several amino acids of L-conformation including asparagine, proline, tryptophane and glutamine as nitrogen sources substantially increased the growth rate of C. limicola. On the other hand phenylalanine, serine, tyrosine and threonine inhibited the growth of bacteria. We also showed that nitric acid salts could not be used as nitrogen source by C. limicola and inhibited uptake of other nitrogen forms. We showed that ammonia deficiency and/or nitrate addition to the medium stimulate the intracellular glycogen accumulation.