Application of green fluorescent protein for monitoring phenol-degrading strains

• Main Authors: Ana Milena Valderrama F., Julia Raquel Acero R.
• Format: Article
• Language: Spanish
• Published: Universidad Nacional de Colombia 2011-12-01
• Series: Revista Colombiana de Biotecnología
• Subjects: GFP; aguas residuales industriales; biorremediación; biosensor
• Online Access: http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/30078

<p class="Default" style="text-align: justify;"><span lang="EN-US">Several methods have been developed for detecting microorganisms in environmental samples. Some systems for incorporating reporter genes, such as <em>lux </em>or the green fluorescent protein (GFP) gene, have been developed recently This study describes <em>gfp </em>gene marking of a phenol degrading strain, its evaluation and monitoring in a bioreactor containing refinery sour water. Tagged strains were obtained having the same physiological and metabolic characteristics as the parent strain. Fluorescent expression was kept stable with no selection for more than 50 consecutive generations and tagged strains were recovered from the bioreactor after forty-five days of phenol-degradation treatment. </span></p>