HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification

HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification

The presented dataset describes the quantification of carbon nanoparticle C60 fullerene accumulated in mitochondria of human leukemic cells treated with nanostructure. Firstly, the high performance liquid chromatography–electro spray ionization–mass spectrometry (HPLC-ESI-MS) method was developed fo...

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Main Authors: Anna Grebinyk, Sergii Grebinyk, Svitlana Prylutska, Uwe Ritter, Olga Matyshevska, Thomas Dandekar, Marcus Frohme
Format: Article
Language:English
Published: Elsevier 2018-08-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340918307406
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spelling doaj-46cf5eda7368431f8be6abe2f60814ae2020-11-25T01:31:38ZengElsevierData in Brief2352-34092018-08-011920472052HPLC-ESI-MS method for C60 fullerene mitochondrial content quantificationAnna Grebinyk0Sergii Grebinyk1Svitlana Prylutska2Uwe Ritter3Olga Matyshevska4Thomas Dandekar5Marcus Frohme6Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Hochschulring 1, 15745 Wildau, Germany; Dept. of Bioinformatics, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany; Educational and Scientific Center ''Institute of Biology and Medicine'', Taras Shevchenko National University of Kyiv, Volodymyrska 64, 01601 Kyiv, UkraineDivision Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Hochschulring 1, 15745 Wildau, GermanyDept. of Chemistry, Taras Shevchenko National University of Kyiv, Volodymyrska 64, 01601 Kyiv, UkraineInstitute of Chemistry and Biotechnology, University of Technology Ilmenau, WeimarerStraße 25 (Curiebau), 98693 Ilmenau, GermanyEducational and Scientific Center ''Institute of Biology and Medicine'', Taras Shevchenko National University of Kyiv, Volodymyrska 64, 01601 Kyiv, UkraineDept. of Bioinformatics, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, GermanyDivision Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Hochschulring 1, 15745 Wildau, Germany; Corresponding author. Fax: +49 (0) 3375 508 261.The presented dataset describes the quantification of carbon nanoparticle C60 fullerene accumulated in mitochondria of human leukemic cells treated with nanostructure. Firstly, the high performance liquid chromatography–electro spray ionization–mass spectrometry (HPLC-ESI-MS) method was developed for quantitative analysis of pristine C60 fullerene. Then, human leukemic cells were incubated with C60 fullerene, homogenized and subjected to the differential centrifugation to retrieve mitochondrial fraction. The C60 fullerene content was quantified by HPLC-ESI-MS in extracts of cellular fractions.This data article refers to the research article “C60 Fullerene Accumulation in Human Leukemic Cells and Perspectives of LED-mediated Photodynamic Therapy” by Grebinyk et al. [1].http://www.sciencedirect.com/science/article/pii/S2352340918307406
collection DOAJ
language English
format Article
sources DOAJ
author Anna Grebinyk
Sergii Grebinyk
Svitlana Prylutska
Uwe Ritter
Olga Matyshevska
Thomas Dandekar
Marcus Frohme
spellingShingle Anna Grebinyk
Sergii Grebinyk
Svitlana Prylutska
Uwe Ritter
Olga Matyshevska
Thomas Dandekar
Marcus Frohme
HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification
Data in Brief
author_facet Anna Grebinyk
Sergii Grebinyk
Svitlana Prylutska
Uwe Ritter
Olga Matyshevska
Thomas Dandekar
Marcus Frohme
author_sort Anna Grebinyk
title HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification
title_short HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification
title_full HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification
title_fullStr HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification
title_full_unstemmed HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification
title_sort hplc-esi-ms method for c60 fullerene mitochondrial content quantification
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2018-08-01
description The presented dataset describes the quantification of carbon nanoparticle C60 fullerene accumulated in mitochondria of human leukemic cells treated with nanostructure. Firstly, the high performance liquid chromatography–electro spray ionization–mass spectrometry (HPLC-ESI-MS) method was developed for quantitative analysis of pristine C60 fullerene. Then, human leukemic cells were incubated with C60 fullerene, homogenized and subjected to the differential centrifugation to retrieve mitochondrial fraction. The C60 fullerene content was quantified by HPLC-ESI-MS in extracts of cellular fractions.This data article refers to the research article “C60 Fullerene Accumulation in Human Leukemic Cells and Perspectives of LED-mediated Photodynamic Therapy” by Grebinyk et al. [1].
url http://www.sciencedirect.com/science/article/pii/S2352340918307406
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