Recombinant yeast VDAC2: a comparison of electrophysiological features with the native form

• Main Authors: Andrea Magrì, Andonis Karachitos, Maria Carmela Di Rosa, Simona Reina, Stefano Conti Nibali, Angela Messina, Hanna Kmita, Vito De Pinto
• Format: Article
• Language: English
• Published: Wiley 2019-07-01
• Series: FEBS Open Bio
• Subjects: electrophysiology; heterologous expression; mitochondria; mitochondrial porins; planar lipid bilayer; VDAC2
• Online Access: https://doi.org/10.1002/2211-5463.12574

Voltage‐dependent anion channel isoform 2 of the yeast Saccharomyces cerevisiae (yVDAC2) was believed for many years to be devoid of channel activity. Recently, we isolated yVDAC2 and showed that it exhibits channel‐forming activity in the planar lipid bilayer system when in its so‐called native form. Here, we describe an alternative strategy for yVDAC2 isolation, through heterologous expression in bacteria and refolding in vitro. Recombinant yVDAC2, like its native form, is able to form voltage‐dependent channels. However, some differences between native and recombinant yVDAC2 emerged in terms of voltage dependence and ion selectivity, suggesting that, in this specific case, the recombinant protein might be depleted of post‐translational modification(s) that occur in eukaryotic cells.