| Summary: | Silver nanoparticles (AgNPs) are employed in a wide range of applications; in this study, we improved the AgNPs manufacturing method using I. carnea methanol leaves extract. We noticed that phytochemicals from I. carnea are capable to reduce silver nitrate and can also act as a capping and stabilizing agent. Between 408 and 412 nm, UV–Vis spectrophotometer absorption spectra (SPR peaks) were discovered. X-ray diffraction (XRD) and Field Emission Scanning Electron Microscope (FE-SEM) analyses revealed that the synthesized AgNPs were spherical face-center-cubic (FCC) crystals with 32.99 nm average particle size. Furthermore, the antibacterial activity of AgNPs was screened against four bacterial pathogen strains, namely Streptococcus pneumoniae (MTCC 655), Staphylococcus aureus (MTCC 1144), Pseudomonas aeruginosa (MTCC 2474), and Klebsiella pneumoniae (MTCC 432), and toxicity was determined using the Brine shrimp lethality assay. With an LC50 value of 1600.66 ng/mL, synthesized AgNPs were shown to be very effective against all MTCC strains.
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